Document Detail

NCAM polysialic acid can regulate both cell-cell and cell-substrate interactions.
Jump to Full Text
MedLine Citation:
PMID:  2050739     Owner:  NLM     Status:  MEDLINE    
We have proposed previously that the polysialic acid (PSA) moiety of NCAM can influence membrane-membrane apposition, and thereby serve as a selective regulator of a variety of contact-dependent cell interactions. In this study, cell and tissue culture models are used to obtain direct evidence that the presence of PSA on the surface membrane can affect both cell-cell and cell-substrate interactions. Using a neuroblastoma/sensory neuron cell hybrid, it was found that removal of PSA with a specific neuraminidase (endo-N) augments cell-cell aggregation mediated by the L1 cell adhesion molecule as well as cell attachment to a variety of tissue culture substrates. In studies of embryonic spinal cord axon bundling, which involves both cell-cell and cell-substrate interactions, the pronounced defasciculation produced by removal of PSA is most easily explained by an increase in cell-substrate interaction. The fact that in both studies NCAM's intrinsic adhesion function was found not to be an important variable further illustrates that regulation of the cell surface by PSA can extend beyond binding mediated by the NCAM polypeptide.
A Acheson; J L Sunshine; U Rutishauser
Related Documents :
19529 - The dispersal of cells from human gynecologic specimens: chemical agents.
8083469 - Positive and negative signals transduced through surface immunoglobulins in human b cells.
22447439 - Spy1 is frequently overexpressed in malignant gliomas and critically regulates the prol...
21557999 - Depletion of cytosolic or mitochondrial thioredoxin increases cyp2e1-induced oxidative ...
21307959 - Correction: perifosine and cci 779 co-operate to induce cell death and decrease prolife...
19929969 - Cell-surface phytase on pichia pastoris cell wall offers great potential as a feed supp...
1968899 - Camp response of cultured sertoli cells from immature and adult hamsters. effect of hyp...
20349949 - Intracellular delivery of the reactive oxygen species generating agent d-penicillamine ...
11539629 - Neoplastic cell transformation by energetic heavy ions and its modification with chemic...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  114     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1991-07-24     Completed Date:  1991-07-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  143-53     Citation Subset:  IM    
Department of Anatomy and Cell Biology, University of Alberta, Edmonton, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Axons / physiology*
Cell Adhesion*
Cell Adhesion Molecules, Neuronal / physiology*
Cell Aggregation
Chick Embryo
Culture Techniques
Hybrid Cells
Laminin / physiology
Models, Biological
Neuraminidase / metabolism
Polysaccharides / physiology*
Sialic Acids / physiology*
Spinal Cord / chemistry
Reg. No./Substance:
0/Cell Adhesion Molecules, Neuronal; 0/Laminin; 0/Polysaccharides; 0/Sialic Acids; 0/polysialic acid; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
Journal ID (publisher-id): J. Cell Biol.
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
Download PDF

Print publication date: Day: 1 Month: 7 Year: 1991
Volume: 114 Issue: 1
First Page: 143 Last Page: 153
ID: 2289064
Publisher Id: 91268147
PubMed Id: 2050739

NCAM polysialic acid can regulate both cell-cell and cell-substrate interactions

Article Categories:
  • Articles

Previous Document:  The function of chitin synthases 2 and 3 in the Saccharomyces cerevisiae cell cycle.
Next Document:  Sphingosine-1-phosphate, a novel lipid, involved in cellular proliferation.