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NCAM polysialic acid can regulate both cell-cell and cell-substrate interactions.
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MedLine Citation:
PMID:  2050739     Owner:  NLM     Status:  MEDLINE    
We have proposed previously that the polysialic acid (PSA) moiety of NCAM can influence membrane-membrane apposition, and thereby serve as a selective regulator of a variety of contact-dependent cell interactions. In this study, cell and tissue culture models are used to obtain direct evidence that the presence of PSA on the surface membrane can affect both cell-cell and cell-substrate interactions. Using a neuroblastoma/sensory neuron cell hybrid, it was found that removal of PSA with a specific neuraminidase (endo-N) augments cell-cell aggregation mediated by the L1 cell adhesion molecule as well as cell attachment to a variety of tissue culture substrates. In studies of embryonic spinal cord axon bundling, which involves both cell-cell and cell-substrate interactions, the pronounced defasciculation produced by removal of PSA is most easily explained by an increase in cell-substrate interaction. The fact that in both studies NCAM's intrinsic adhesion function was found not to be an important variable further illustrates that regulation of the cell surface by PSA can extend beyond binding mediated by the NCAM polypeptide.
A Acheson; J L Sunshine; U Rutishauser
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of cell biology     Volume:  114     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1991-07-24     Completed Date:  1991-07-24     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  143-53     Citation Subset:  IM    
Department of Anatomy and Cell Biology, University of Alberta, Edmonton, Canada.
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MeSH Terms
Axons / physiology*
Cell Adhesion*
Cell Adhesion Molecules, Neuronal / physiology*
Cell Aggregation
Chick Embryo
Culture Techniques
Hybrid Cells
Laminin / physiology
Models, Biological
Neuraminidase / metabolism
Polysaccharides / physiology*
Sialic Acids / physiology*
Spinal Cord / chemistry
Reg. No./Substance:
0/Cell Adhesion Molecules, Neuronal; 0/Laminin; 0/Polysaccharides; 0/Sialic Acids; 0/polysialic acid; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
Journal ID (publisher-id): J. Cell Biol.
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 7 Year: 1991
Volume: 114 Issue: 1
First Page: 143 Last Page: 153
ID: 2289064
Publisher Id: 91268147
PubMed Id: 2050739

NCAM polysialic acid can regulate both cell-cell and cell-substrate interactions

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