| NADPH oxidase-mediated generation of reactive oxygen species: A new mechanism for X-ray-induced HeLa cell death. | |
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MedLine Citation:
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PMID: 18952061 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxidative damage is an important mechanism in X-ray-induced cell death. Radiolysis of water molecules is a source of reactive oxygen species (ROS) that contribute to X-ray-induced cell death. In this study, we showed by ROS detection and a cell survival assay that NADPH oxidase has a very important role in X-ray-induced cell death. Under X-ray irradiation, the upregulation of the expression of NADPH oxidase membrane subunit gp91(phox) was dose-dependent. Meanwhile, the cytoplasmic subunit p47(phox) was translocated to the cell membrane and localized with p22(phox) and gp91(phox) to form reactive NADPH oxidase. Our data suggest, for the first time, that NADPH oxidase-mediated generation of ROS is an important contributor to X-ray-induced cell death. This suggests a new target for combined gene transfer and radiotherapy. |
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Authors:
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Qing Liu; Xiaoqing He; Yongsheng Liu; Bingbing Du; Xiaoyan Wang; Weisheng Zhang; Pengfei Jia; Jingmei Dong; Jianxiu Ma; Xiaohu Wang; Sha Li; Hong Zhang |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-10-24 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 377 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-11-25 Completed Date: 2008-12-15 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 775-9 Citation Subset: IM |
Affiliation:
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Department of Radiobiology, Institute of Modern Physics, Chinese Academy of Sciences, 509 Nanchang Rd., Lanzhou 730000, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis* Gene Therapy Hela Cells Humans Membrane Glycoproteins / metabolism NADPH Oxidase / genetics, metabolism* Neoplasms / radiotherapy, therapy Radiation Tolerance / genetics Reactive Oxygen Species / metabolism* X-Rays* |
| Chemical | |
Reg. No./Substance:
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0/CYBB protein, human; 0/Membrane Glycoproteins; 0/Reactive Oxygen Species; EC 1.6.3.1/CYBA protein, human; EC 1.6.3.1/NADPH Oxidase; EC 1.6.3.1/neutrophil cytosolic factor 1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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