| NADPH oxidase-mediated generation of reactive oxygen species is critically required for survival of undifferentiated human promyelocytic leukemia cell line HL-60. | |
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MedLine Citation:
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PMID: 15346654 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase) mediated generation of reactive oxygen species (ROS) was originally identified as the powerful host defense machinery against microorganism in phagocytes. But recent reports indicated that some non-phagocytic cells also have the NADPH oxidase activity, and the ROS produced by it may act as cell signal molecule. But as far as today, whether the NADPH oxidase also plays similar role in phagocyte has not been paid much attention. Utilizing the undifferentiated HL-60 promyelocytic leukemia cells as a model, the aim of the present study was to determine whether NADPH oxidase plays a role on ROS generation in undifferentiated HL-60, and the ROS mediated by it was essential for cell's survival. For the first time, we verified that the release of ROS in undifferentiated HL-60 was significantly increased by the stimulation with Calcium ionophore or opsonized zymosan, which are known to trigger respiration burst in phagocytes by NADPH oxidase pathway. Diphenylene iodonium (DPI) or apocynin (APO), two inhibitors of NADPH oxidase, significantly suppressed the increasing of ROS caused by opsonized zymosan. Cell survival assay and fluorescence double dyeing with acridine orange and ethidium bromide showed that DPI and APO, as well as superoxide dismutase (SOD) and catalase (CAT) concentration-dependently decreased the viability of undifferentiated HL-60 cells, whereas exogenous H2O2 can rescue the cells from death obviously. Our results suggested that the ROS, generated by NADPH oxidase play an essential role in the survival of undifferentiated HL-60 cells. |
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Authors:
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Jing-Mei Dong; Sheng-Guo Zhao; Guo-Yin Huang; Qing Liu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Free radical research Volume: 38 ISSN: 1071-5762 ISO Abbreviation: Free Radic. Res. Publication Date: 2004 Jun |
Date Detail:
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Created Date: 2004-09-06 Completed Date: 2004-11-23 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: England |
Other Details:
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Languages: eng Pagination: 629-37 Citation Subset: IM |
Affiliation:
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Department of Physical, Lanzhou Teacher's College, Lanzhou, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetophenones
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pharmacology Cell Survival / drug effects HL-60 Cells Humans Hydrogen Peroxide / metabolism Ionophores / pharmacology Leukemia, Promyelocytic, Acute / drug therapy, metabolism* NADPH Oxidase / metabolism* Onium Compounds / pharmacology Reactive Oxygen Species / metabolism* Time Factors Zymosan / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Acetophenones; 0/Ionophores; 0/Onium Compounds; 0/Reactive Oxygen Species; 244-54-2/diphenyleneiodonium; 498-02-2/acetovanillone; 7722-84-1/Hydrogen Peroxide; 9010-72-4/Zymosan; EC 1.6.3.1/NADPH Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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