| NAD(P)H oxidase isoform Nox2 plays a prosurvival role in human leukaemia cells. | |
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MedLine Citation:
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PMID: 19707918 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mechanism involved in the prosurvival effect of interleukin-3 on the human acute myeloid leukaemia cell line M07e is investigated. A decrease in intracellular reactive oxygen species (ROS) content, glucose transport activity and cell survival was observed in the presence of inhibitors of plasma membrane ROS sources, such as diphenylene iodonium and apocynin, and by small interference RNA for Nox2. Moreover, IL-3 incubation stimulated the synthesis of Nox2 cytosolic sub-unit p47phox and glucose transporter Glut1. Thus, the inhibition of ROS generation by Nox inhibitors stimulated apoptosis showing that ROS production, induced by IL-3 via Nox2, protects leukaemic cells from cell death. Also incubation with receptor tyrosine kinase inhibitors, such as anti-leukaemic drugs blocking the stem cell factor receptor (c-kit), showed similar effects, hinting that IL-3 transmodulates c-kit phosphorylation. These mechanisms may play an important role in acute myeloid leukaemia treatment, representing a novel therapeutic target. |
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Authors:
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Tullia Maraldi; Cecilia Prata; Francesco Vieceli Dalla Sega; Cristiana Caliceti; Laura Zambonin; Diana Fiorentini; Gabriele Hakim |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Free radical research Volume: 43 ISSN: 1029-2470 ISO Abbreviation: Free Radic. Res. Publication Date: 2009 |
Date Detail:
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Created Date: 2010-06-17 Completed Date: 2010-10-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9423872 Medline TA: Free Radic Res Country: England |
Other Details:
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Languages: eng Pagination: 1111-21 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Histology, University of Modena and Reggio Emilia, Modena, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Biological Transport Cell Line, Tumor Cell Survival / physiology Glucose Transporter Type 1 / metabolism Humans Interleukin-3 / metabolism Leukemia, Myeloid, Acute / enzymology*, pathology Membrane Glycoproteins / metabolism* NADPH Oxidase / metabolism* Phosphorylation / drug effects Reactive Oxygen Species / metabolism |
| Chemical | |
Reg. No./Substance:
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0/CYBB protein, human; 0/Glucose Transporter Type 1; 0/Interleukin-3; 0/Membrane Glycoproteins; 0/Reactive Oxygen Species; EC 1.6.3.1/NADPH Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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