Document Detail


NADP-dehydrogenases from pepper fruits: effect of maturation.
MedLine Citation:
PMID:  19055545     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
NADPH is an important molecule in the redox balance of the cell. Pepper fruits are the second worldwide consumable vegetables and exhibit different phenotypes after maturation. In this paper, two pepper cultivars were studied: Vergasa whose fruits shift from green to red after maturation, and Biela that shifts to yellow. Using fresh fruits from the same plants of the two cultivars at distinct maturation stages, the activity and gene expression of the main NADPH-generating dehydrogenases was studied. The activity analysis of the main NADP-dehydrogenases, glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconate dehydrogenase (6PGDH), NADP-isocitrate dehydrogenase (NADP-ICDH) and NADP-malic enzyme (NADP-ME), showed that, except for the G6PDH, all the activities were enhanced (54-100%) in the mature pepper fruits from both cultivars (red or yellow) with respect to green pepper fruits. The content of NADPH and NADP in the mature fruits of both cultivars showed a noteworthy increase with respect to green fruits. For the transcript analysis, a partial cDNA of each NADP-dehydrogenase was obtained, and the NADP-ME was the only NADP-dehydrogenase that showed a significant induction. The increase in the content of NADPH in mature fruits because of the enhanced activity of NADP-dehydrogenases suggests that these NADPH-generating enzymes could be involved in the maturation of pepper fruits.
Authors:
Rosa M Mateos; Daniel Bonilla-Valverde; Luis A del Río; José M Palma; Francisco J Corpas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-11-14
Journal Detail:
Title:  Physiologia plantarum     Volume:  135     ISSN:  1399-3054     ISO Abbreviation:  Physiol Plant     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-01-20     Completed Date:  2009-02-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1256322     Medline TA:  Physiol Plant     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  130-9     Citation Subset:  IM    
Affiliation:
Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidín, CSIC, Granada, Spain.
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MeSH Terms
Descriptor/Qualifier:
Capsicum / enzymology*,  genetics
DNA, Complementary / genetics
Fruit / enzymology*,  genetics
Glucosephosphate Dehydrogenase / metabolism
Isocitrate Dehydrogenase / metabolism
Malate Dehydrogenase / metabolism
NADP / metabolism
NADPH Dehydrogenase / genetics,  metabolism*
Phosphogluconate Dehydrogenase / metabolism
RNA, Plant / genetics
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/RNA, Plant; 53-59-8/NADP; EC 1.1.1.37/Malate Dehydrogenase; EC 1.1.1.40/malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+); EC 1.1.1.41/Isocitrate Dehydrogenase; EC 1.1.1.42/isocitrate dehydrogenase (NADP+); EC 1.1.1.43/Phosphogluconate Dehydrogenase; EC 1.1.1.49/Glucosephosphate Dehydrogenase; EC 1.6.99.1/NADPH Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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