| NADH oxidation by manganese peroxidase with or without alpha-hydroxy acid. | |
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MedLine Citation:
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PMID: 12036041 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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NADH oxidation by manganese peroxidase (MnP) was done in a reaction mixture including either alpha-hydroxy acid or acetate. The oxidation in the former reaction mixture was inhibited by a catalase and was accelerated by exogenous H2O2, while the oxidation in the latter reaction mixture was inhibited by a superoxide dismutase and was not accelerated by the exogenous H2O2. These results indicated that there are significant differences between the two reaction systems, particularly, in the active oxygen species involved in the reactions. Additionally, the experiment of MnP reduction with Mn(II) suggests that MnP has a separate catalytic activity other than an oxidation of Mn(II) to Mn(III) in the reaction mixture including acetate. |
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Authors:
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Tetsuya Deguchi; Masaaki Matsubara; Tomoaki Nishida |
Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 66 ISSN: 0916-8451 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2002 Apr |
Date Detail:
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Created Date: 2002-05-30 Completed Date: 2002-11-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 717-21 Citation Subset: IM |
Affiliation:
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Chemical and Environmental Technology Laboratory, Kobe Steel, Ltd., Japan. te-deguchi@rd.kcrl.kobelco.co.jp |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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pharmacology Fungi / enzymology* Hydrogen Peroxide / pharmacology Hydroxy Acids / pharmacology* Kinetics Malates / pharmacology NAD / metabolism* Oxidation-Reduction Peroxidases / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Hydroxy Acids; 0/Malates; 53-84-9/NAD; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.-/Peroxidases; EC 1.11.1.13/manganese peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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