Document Detail


NADH distribution in live progenitor stem cells by phasor-fluorescence lifetime image microscopy.
MedLine Citation:
PMID:  22828352     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
NADH is a naturally fluorescent metabolite associated with cellular respiration. Exploiting the different fluorescence lifetime of free and bound NADH has the potential to quantify the relative amount of bound and free NADH, enhancing understanding of cellular processes including apoptosis, cancer pathology, and enzyme kinetics. We use the phasor-fluorescence lifetime image microscopy approach to spatially map NADH in both the free and bound forms of live undifferentiated and differentiated myoblast cells. The phasor approach graphically depicts the change in lifetime at a pixel level without the requirement for fitting the decay. Comparison of the spatial distribution of NADH in the nucleus of cells induced to differentiate through serum starvation and undifferentiated cells show differing distributions of bound and free NADH. Undifferentiated cells displayed a short lifetime indicative of free NADH in the nucleus and a longer lifetime attributed to the presence of bound NADH outside of the nucleus. Differentiating cells displayed redistribution of free NADH with decreased relative concentration of free NADH within the nucleus whereas the majority of NADH was found in the cytoplasm.
Authors:
Belinda K Wright; Laura M Andrews; Julie Markham; Mark R Jones; Chiara Stringari; Michelle A Digman; Enrico Gratton
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Biophysical journal     Volume:  103     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-25     Completed Date:  2012-12-10     Revised Date:  2013-07-30    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  L7-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Biophysical Society. Published by Elsevier Inc. All rights reserved.
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MeSH Terms
Descriptor/Qualifier:
Animals
Microscopy, Fluorescence
Myoblasts / chemistry*
NAD / analysis*
Rats
Grant Support
ID/Acronym/Agency:
P30 CA062203/CA/NCI NIH HHS; P41 GM103540/GM/NIGMS NIH HHS; P41 GM103540/GM/NIGMS NIH HHS; P41 RR003155/RR/NCRR NIH HHS; P41-RR03155/RR/NCRR NIH HHS; P50 GM076516/GM/NIGMS NIH HHS; P50 GM076516/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
53-84-9/NAD
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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