Document Detail


NAD biosynthesis: identification of the tryptophan to quinolinate pathway in bacteria.
MedLine Citation:
PMID:  14700627     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Previous studies have demonstrated two different biosynthetic pathways to quinolinate, the universal de novo precursor to the pyridine ring of NAD. In prokaryotes, quinolinate is formed from aspartate and dihydroxyacetone phosphate; in eukaryotes, it is formed from tryptophan. It has been generally believed that the tryptophan to quinolinic acid biosynthetic pathway is unique to eukaryotes; however, this paper describes the use of comparative genome analysis to identify likely candidates for all five genes involved in the tryptophan to quinolinic acid pathway in several bacteria. Representative examples of each of these genes were overexpressed, and the predicted functions are confirmed in each case using unambiguous biochemical assays.
Authors:
Oleg Kurnasov; Vasiliy Goral; Keri Colabroy; Svetlana Gerdes; Shubha Anantha; Andrei Osterman; Tadhg P Begley
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Chemistry & biology     Volume:  10     ISSN:  1074-5521     ISO Abbreviation:  Chem. Biol.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-31     Completed Date:  2004-09-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9500160     Medline TA:  Chem Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1195-204     Citation Subset:  IM    
Affiliation:
Integrated Genomics, 2201 West Campbell Park Drive, Chicago, IL 60612, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AY304519;  AY305284;  AY305285;  AY308027;  AY308028;  AY308029;  AY308030;  AY308031;  AY308032;  AY308033;  AY308034;  AY308035;  AY308036;  AY308037;  AY308038;  AY308039
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MeSH Terms
Descriptor/Qualifier:
Arylformamidase / genetics,  metabolism
Bacteria / enzymology*,  genetics*,  metabolism
Chromosomes, Bacterial / genetics
Gene Expression Regulation, Bacterial
Genes, Bacterial / genetics
Genetic Complementation Test
Hydrolases / genetics,  metabolism
Kinetics
Molecular Sequence Data
NAD / biosynthesis*,  chemistry
Oxidoreductases / genetics,  metabolism
Quinolinic Acid / chemistry,  metabolism*
Tryptophan / chemistry,  metabolism*
Grant Support
ID/Acronym/Agency:
DK44083/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
53-84-9/NAD; 73-22-3/Tryptophan; 89-00-9/Quinolinic Acid; EC 1.-/Oxidoreductases; EC 3.-/Hydrolases; EC 3.5.1.9/Arylformamidase; EC 3.7.1.3/kynureninase
Comments/Corrections
Comment In:
Chem Biol. 2003 Dec;10(12):1141-2   [PMID:  14700618 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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