Document Detail

NAD biosynthesis: identification of the tryptophan to quinolinate pathway in bacteria.
MedLine Citation:
PMID:  14700627     Owner:  NLM     Status:  MEDLINE    
Previous studies have demonstrated two different biosynthetic pathways to quinolinate, the universal de novo precursor to the pyridine ring of NAD. In prokaryotes, quinolinate is formed from aspartate and dihydroxyacetone phosphate; in eukaryotes, it is formed from tryptophan. It has been generally believed that the tryptophan to quinolinic acid biosynthetic pathway is unique to eukaryotes; however, this paper describes the use of comparative genome analysis to identify likely candidates for all five genes involved in the tryptophan to quinolinic acid pathway in several bacteria. Representative examples of each of these genes were overexpressed, and the predicted functions are confirmed in each case using unambiguous biochemical assays.
Oleg Kurnasov; Vasiliy Goral; Keri Colabroy; Svetlana Gerdes; Shubha Anantha; Andrei Osterman; Tadhg P Begley
Related Documents :
18436597 - Frequency and expression of mutacin biosynthesis genes in isolates of streptococcus mut...
16441377 - Disruption of ste23 gene affects composition profile and bioactivity of exopolysacchari...
25286177 - Sequencing human rhinoviruses: direct sequencing versus plasmid cloning.
12530217 - Sequence of a cdna and expression of the gene encoding a putative epidermal chitin synt...
20214897 - Haemonchus contortus: cloning and characterization of serpin.
14575087 - Relationship of pre-1918 avian influenza ha and np sequences to subsequent avian influe...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Chemistry & biology     Volume:  10     ISSN:  1074-5521     ISO Abbreviation:  Chem. Biol.     Publication Date:  2003 Dec 
Date Detail:
Created Date:  2003-12-31     Completed Date:  2004-09-16     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9500160     Medline TA:  Chem Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1195-204     Citation Subset:  IM    
Integrated Genomics, 2201 West Campbell Park Drive, Chicago, IL 60612, USA.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AY304519;  AY305284;  AY305285;  AY308027;  AY308028;  AY308029;  AY308030;  AY308031;  AY308032;  AY308033;  AY308034;  AY308035;  AY308036;  AY308037;  AY308038;  AY308039
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Arylformamidase / genetics,  metabolism
Bacteria / enzymology*,  genetics*,  metabolism
Chromosomes, Bacterial / genetics
Gene Expression Regulation, Bacterial
Genes, Bacterial / genetics
Genetic Complementation Test
Hydrolases / genetics,  metabolism
Molecular Sequence Data
NAD / biosynthesis*,  chemistry
Oxidoreductases / genetics,  metabolism
Quinolinic Acid / chemistry,  metabolism*
Tryptophan / chemistry,  metabolism*
Grant Support
Reg. No./Substance:
53-84-9/NAD; 73-22-3/Tryptophan; 89-00-9/Quinolinic Acid; EC 1.-/Oxidoreductases; EC 3.-/Hydrolases; EC; EC
Comment In:
Chem Biol. 2003 Dec;10(12):1141-2   [PMID:  14700618 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Inhibitor specificity via protein dynamics: insights from the design of antibacterial agents targete...
Next Document:  A new H/D exchange- and mass spectrometry-based method for thermodynamic analysis of protein-DNA int...