Document Detail

NAD+ biosynthesis from tryptophan in the presence of nicotinic acid or vice versa by rat hepatocytes--effect of clofibrate-feeding.
MedLine Citation:
PMID:  9565825     Owner:  NLM     Status:  MEDLINE    
NAD+ biosynthesis from tryptophan in the presence of nicotinic acid or vice versa by rat hepatocytes was investigated. In the control hepatocytes, NAD+ synthesis from tryptophan was not affected by nicotinic acid from 0.026 to 0.26 mM. NAD+ synthesis from nicotinic acid was slightly inhibited with varying concentrations of tryptophan from 0.1 to 1.0 mM. In the clofibrate-treated hepatocytes, NAD+ synthesis from tryptophan was greatly increased (234% of the control), while that from nicotinic acid was decreased (71.2% of the control). Both, NAD+ synthesis from tryptophan and that from nicotinic acid were decreased by the coexisting nicotinic acid or tryptophan. Total amount of NAD+ synthesized from tryptophan and nicotinic acid at their physiological concentrations was significantly higher than that in the control hepatocytes as a result of a large increase of NAD+ synthesized from tryptophan. When the metabolic flux of 0.1 or 0.5mM tryptophan was investigated, the glutarate pathway was suppressed in the clofibrate-treated hepatocytes, the quinolinic acid-NAD+ flux being elevated. Similarly to clofibrate, DEHP and CPP revealed an increase in NAD+ synthesis from tryptophan. Mutual relationship of NAD+ biosyntheses from tryptophan and nicotinic acid in rat hepatocytes is discussed and the relevance with peroxisomal proliferation is suggested.
M Shin; S Nakakita; C Hashimoto; K Sano; C Umezawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition     Volume:  68     ISSN:  0300-9831     ISO Abbreviation:  Int J Vitam Nutr Res     Publication Date:  1998  
Date Detail:
Created Date:  1998-06-08     Completed Date:  1998-06-08     Revised Date:  2007-02-21    
Medline Journal Info:
Nlm Unique ID:  1273304     Medline TA:  Int J Vitam Nutr Res     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  104-8     Citation Subset:  IM    
School of Pharmacy, Kobe Gakuin University, Japan.
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MeSH Terms
Biological Transport
Clofibrate / pharmacology*
Glutarates / metabolism
Hydrolases / metabolism
Liver / drug effects,  metabolism*
Microbodies / drug effects
NAD / biosynthesis*
Niacin / metabolism*,  pharmacology
Rats, Sprague-Dawley
Tryptophan / metabolism*,  pharmacology
Reg. No./Substance:
0/Glutarates; 110-94-1/glutaric acid; 53-84-9/NAD; 59-67-6/Niacin; 637-07-0/Clofibrate; 73-22-3/Tryptophan; EC 3.-/Hydrolases; EC

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