| N-nitroso compounds in the gastrointestinal tract of rats and in the feces of mice with induced colitis or fed hot dogs or beef. | |
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MedLine Citation:
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PMID: 12663523 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Because colonic N-nitroso compounds (NOC) may be a cause of colon cancer, we determined total NOC levels by Walters' method in the gastrointestinal tract and feces of rodents: (i) feces of C57BL mice fed chow and semi-purified diets contained 3.2 +/- 0.4 and 0.46 +/- 0.06 NOC/g, respectively (P < 0.01, mean +/- SD). (ii) NOC levels for gastrointestinal contents of three groups of Sprague-Dawley rats fed chow diet were 0.9 +/- 0.05 (diet), 0.2 +/- 0 (stomach), 0.3-0.4 (small intestine), 0.7-1.6 (cecum and colon) and 2.6 +/- 0.6 (feces) nmol/g. NOC precursor (NOCP) levels (measured as NOC after mild nitrosation) for two rat groups fed chow diet showed a 16-fold increase from stomach to proximal small intestine (mean, 6.2 micromol/g), and a 1.7-fold increase from distal colon to feces (mean, 11.6 micromol/g). (iii) Eight Min and five C57BL/6J mice received 4% dextran sulfate sodium in drinking water on days 1-4 to induce acute colitis. This increased fecal NOC levels 1.9-fold on day 5 in both strains (P < or = 0.04), probably due to NO synthase-derived nitrosating agents in the colon. (iv) Following studies on humans fed beef [Hughes et al. (2001) Carcinogenesis, 22, 199], Swiss mice received semi-purified diets mixed with 18% of beef plus pork hot dogs or sautéed beef for 7 days. On day 7, individual 24-h fecal NOC outputs were determined. In three hot dog and two beef groups with 5 mice/group, mean fecal NOC output/day was 3.7-5.0 (hot dog) and 2.0-2.9 (beef) times that for control groups fed semi-purified diet alone (P < 0.002 for each of combined groups). These groups showed little change in fecal NOCP output. (v) Initial purification of rat fecal NOCP by adsorption-desorption and HPLC is described. Results should help evaluate the view that colonic NOC causes colon cancer associated with colitis and ingestion of red and nitrite-preserved meat. |
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Authors:
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Sidney S Mirvish; James Haorah; Lin Zhou; Melissa Hartman; Chantey R Morris; Marge L Clapper |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Carcinogenesis Volume: 24 ISSN: 0143-3334 ISO Abbreviation: Carcinogenesis Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-03-28 Completed Date: 2003-04-30 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: England |
Other Details:
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Languages: eng Pagination: 595-603 Citation Subset: IM |
Affiliation:
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Eppley Institute for Research in Cancer and Department of Pharmaceutical Sciences , University of Nebraska Medical Center, Omaha, NE 68198, USA. smirvish@unmc.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Digestive System / metabolism* Feces / chemistry* Male Meat Products* Mice Mice, Inbred C57BL Nitroso Compounds / analysis, metabolism* Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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CA-06927/CA/NCI NIH HHS; P30-CA-36727/CA/NCI NIH HHS; R01-CA-71483/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Nitroso Compounds |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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