Document Detail


N-methyl-D-aspartate receptor antagonist ketamine selectively attenuates spontaneous phasic activity of supraoptic vasopressin neurons in vivo.
MedLine Citation:
PMID:  7514767     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Supraoptic neurosecretory neurons express a prominent N-methyl-D-aspartate receptor system. Recent in vitro evidence reveals that N-methyl-D-aspartate receptor activation dramatically alters the spontaneous discharge patterns of supraoptic neurons. In this study we evaluate whether N-methyl-D-aspartate receptors in vivo contribute to the development of characteristic phasic discharge patterns displayed by vasopressin-secreting neurons. Intravenous administration of ketamine hydrochloride, a non-competitive N-methyl-D-aspartate receptor antagonist, was used to examine whether N-methyl-D-aspartate receptor blockade influences patterned spontaneous discharge observed in supraoptic neurosecretory neurons. Extracellular recordings were obtained from identified hypothalamic supraoptic neurons in pentobarbital-anaesthetized Long-Evans rats. Systemic administration of ketamine (< or = 1.5 mg/kg) potently suppressed spontaneous phasic discharge in 16/19 putative vasopressin-secreting cells. The ketamine-induced blockade was dose dependent, fully reversible and was associated with the complete blockade of activity evoked by local pressure application of N-methyl-D-aspartate, but not the activity evoked by alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionate receptor agonists (6/6 cells). Ketamine had no detectable effect on threshold or shape of antidromic action potentials. By comparison, the activity in 9/10 continuously active neurons (putative oxytocin-secreting) was unaffected by administration of identical doses of ketamine. These data suggest that N-methyl-D-aspartate receptors play an important role in regulating the onset and maintenance of spontaneous phasic activity patterns displayed by rat supraoptic vasopressin neurons in vivo.
Authors:
R Nissen; B Hu; L P Renaud
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Neuroscience     Volume:  59     ISSN:  0306-4522     ISO Abbreviation:  Neuroscience     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-06-23     Completed Date:  1994-06-23     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7605074     Medline TA:  Neuroscience     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  115-20     Citation Subset:  IM    
Affiliation:
Neuroscience Unit, Loeb Research Institute, Ottawa Civic Hospital, Ontario, Canada.
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MeSH Terms
Descriptor/Qualifier:
Animals
Electrophysiology
Injections, Intravenous
Ketamine / pharmacology*
Male
Neurons / drug effects,  physiology*
Rats
Rats, Inbred Strains
Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors*
Supraoptic Nucleus / physiology*
Vasopressins / physiology*
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / pharmacology
Chemical
Reg. No./Substance:
0/Receptors, N-Methyl-D-Aspartate; 11000-17-2/Vasopressins; 6740-88-1/Ketamine; 77521-29-0/alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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