| N-methyl-D-aspartate receptor antagonist ketamine selectively attenuates spontaneous phasic activity of supraoptic vasopressin neurons in vivo. | |
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MedLine Citation:
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PMID: 7514767 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Supraoptic neurosecretory neurons express a prominent N-methyl-D-aspartate receptor system. Recent in vitro evidence reveals that N-methyl-D-aspartate receptor activation dramatically alters the spontaneous discharge patterns of supraoptic neurons. In this study we evaluate whether N-methyl-D-aspartate receptors in vivo contribute to the development of characteristic phasic discharge patterns displayed by vasopressin-secreting neurons. Intravenous administration of ketamine hydrochloride, a non-competitive N-methyl-D-aspartate receptor antagonist, was used to examine whether N-methyl-D-aspartate receptor blockade influences patterned spontaneous discharge observed in supraoptic neurosecretory neurons. Extracellular recordings were obtained from identified hypothalamic supraoptic neurons in pentobarbital-anaesthetized Long-Evans rats. Systemic administration of ketamine (< or = 1.5 mg/kg) potently suppressed spontaneous phasic discharge in 16/19 putative vasopressin-secreting cells. The ketamine-induced blockade was dose dependent, fully reversible and was associated with the complete blockade of activity evoked by local pressure application of N-methyl-D-aspartate, but not the activity evoked by alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionate receptor agonists (6/6 cells). Ketamine had no detectable effect on threshold or shape of antidromic action potentials. By comparison, the activity in 9/10 continuously active neurons (putative oxytocin-secreting) was unaffected by administration of identical doses of ketamine. These data suggest that N-methyl-D-aspartate receptors play an important role in regulating the onset and maintenance of spontaneous phasic activity patterns displayed by rat supraoptic vasopressin neurons in vivo. |
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Authors:
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R Nissen; B Hu; L P Renaud |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Neuroscience Volume: 59 ISSN: 0306-4522 ISO Abbreviation: Neuroscience Publication Date: 1994 Mar |
Date Detail:
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Created Date: 1994-06-23 Completed Date: 1994-06-23 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 7605074 Medline TA: Neuroscience Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 115-20 Citation Subset: IM |
Affiliation:
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Neuroscience Unit, Loeb Research Institute, Ottawa Civic Hospital, Ontario, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Electrophysiology Injections, Intravenous Ketamine / pharmacology* Male Neurons / drug effects, physiology* Rats Rats, Inbred Strains Receptors, N-Methyl-D-Aspartate / antagonists & inhibitors* Supraoptic Nucleus / physiology* Vasopressins / physiology* alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Receptors, N-Methyl-D-Aspartate; 11000-17-2/Vasopressins; 6740-88-1/Ketamine; 77521-29-0/alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid |
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