Document Detail


N-hexanoyl chitosan-stabilized magnetic nanoparticles: enhancement of adenoviral-mediated gene expression both in vitro and in vivo.
MedLine Citation:
PMID:  18374634     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We developed hexanoyl chloride-modified chitosan (Nac-6) stabilized iron oxide nanoparticles (Nac-6-IOPs) as magnetic nanoparticles for viral gene (Ad/LacZ) delivery via magnetofection. This vector, Nac-6-IOPs/Ad/LacZ, binds to K562 cells in the presence of external magnetic fields and results in enhanced expression of the transgene in those cells that do not exhibit the coxsackie-adenovirus receptor (CAR). Our results demonstrate that Nac-6-IOPs/Ad/LacZ is able to transduce K562 cells specifically with reduced infection of CAR- cells. The dramatic enhancement in intracellular trafficking of the adenovirus without genetically modified vesicles can lead to enhanced nuclear transfer, especially in CAR- cells. In vivo magnetofection results also clearly demonstrated that the present Nac-6-IOPs could be applied in other cell lines. Whether cells or organs, in the presence of magnetic fields Nac-6-IOPs/Ad/LacZ has high transduction efficiency. The newly formulated Nac-6-IOPs, introduced by magnetofection, provide a high-throughput gene screening both in vitro and in vivo.
Authors:
Shanta Raj Bhattarai; Sun Young Kim; Kyu Yun Jang; Ki Chang Lee; Ho Keun Yi; Dae Yeol Lee; Hak Yong Kim; Pyoung Han Hwang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-18
Journal Detail:
Title:  Nanomedicine : nanotechnology, biology, and medicine     Volume:  4     ISSN:  1549-9642     ISO Abbreviation:  -     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-03     Completed Date:  2008-07-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101233142     Medline TA:  Nanomedicine     Country:  United States    
Other Details:
Languages:  eng     Pagination:  146-54     Citation Subset:  IM    
Affiliation:
Department of Pharmaceutical Sciences, Wayne State University, Detroit, Michigan, USA.
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MeSH Terms
Descriptor/Qualifier:
Adenoviridae / genetics*
Animals
Cell Line, Tumor
Chitosan / chemistry*
DNA, Viral / administration & dosage*,  chemistry
Drug Carriers / chemistry*
Female
Gene Expression / physiology
Humans
K562 Cells
Magnetics*
Materials Testing
Mice
Mice, Inbred BALB C
Nanoparticles / chemistry*
Transfection / methods*
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/Drug Carriers; 9012-76-4/Chitosan

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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