Document Detail

N-Glycosylation instead of cholesterol mediates oligomerization and apical sorting of GPI-APs in FRT cells.
MedLine Citation:
PMID:  21998201     Owner:  NLM     Status:  MEDLINE    
Sorting of glycosylphosphatidyl-inositol--anchored proteins (GPI-APs) in polarized epithelial cells is not fully understood. Oligomerization in the Golgi complex has emerged as the crucial event driving apical segregation of GPI-APs in two different kind of epithelial cells, Madin-Darby canine kidney (MDCK) and Fisher rat thyroid (FRT) cells, but whether the mechanism is conserved is unknown. In MDCK cells cholesterol promotes GPI-AP oligomerization, as well as apical sorting of GPI-APs. Here we show that FRT cells lack this cholesterol-driven oligomerization as apical sorting mechanism. In these cells both apical and basolateral GPI-APs display restricted diffusion in the Golgi likely due to a cholesterol-enriched membrane environment. It is striking that N-glycosylation is the critical event for oligomerization and apical sorting of GPI-APs in FRT cells but not in MDCK cells. Our data indicate that at least two mechanisms exist to determine oligomerization in the Golgi leading to apical sorting of GPI-APs. One depends on cholesterol, and the other depends on N-glycosylation and is insensitive to cholesterol addition or depletion.
Naga Salaija Imjeti; Stéphanie Lebreton; Simona Paladino; Erwin de la Fuente; Alfonso Gonzalez; Chiara Zurzolo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-10-12
Journal Detail:
Title:  Molecular biology of the cell     Volume:  22     ISSN:  1939-4586     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-30     Completed Date:  2012-04-12     Revised Date:  2013-06-27    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4621-34     Citation Subset:  IM    
Institut Pasteur, Unité de Traffic Membranaire et Pathogenèse, 75015 Paris, France.
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MeSH Terms
Cell Polarity
Cells, Cultured
Cholesterol / metabolism*,  pharmacology
Epithelial Cells / cytology,  drug effects,  metabolism*
Fluorescence Recovery After Photobleaching
GPI-Linked Proteins / genetics,  metabolism*
Golgi Apparatus / metabolism,  ultrastructure
Green Fluorescent Proteins / genetics,  metabolism
Kidney / cytology
Protein Transport
Rats, Inbred F344
Recombinant Fusion Proteins / genetics,  metabolism
Thyroid Gland / cytology,  metabolism
Reg. No./Substance:
0/GPI-Linked Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; 57-88-5/Cholesterol

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