| N-Glycosylation instead of cholesterol mediates oligomerization and apical sorting of GPI-APs in FRT cells. | |
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MedLine Citation:
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PMID: 21998201 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sorting of glycosylphosphatidyl-inositol--anchored proteins (GPI-APs) in polarized epithelial cells is not fully understood. Oligomerization in the Golgi complex has emerged as the crucial event driving apical segregation of GPI-APs in two different kind of epithelial cells, Madin-Darby canine kidney (MDCK) and Fisher rat thyroid (FRT) cells, but whether the mechanism is conserved is unknown. In MDCK cells cholesterol promotes GPI-AP oligomerization, as well as apical sorting of GPI-APs. Here we show that FRT cells lack this cholesterol-driven oligomerization as apical sorting mechanism. In these cells both apical and basolateral GPI-APs display restricted diffusion in the Golgi likely due to a cholesterol-enriched membrane environment. It is striking that N-glycosylation is the critical event for oligomerization and apical sorting of GPI-APs in FRT cells but not in MDCK cells. Our data indicate that at least two mechanisms exist to determine oligomerization in the Golgi leading to apical sorting of GPI-APs. One depends on cholesterol, and the other depends on N-glycosylation and is insensitive to cholesterol addition or depletion. |
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Authors:
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Naga Salaija Imjeti; Stéphanie Lebreton; Simona Paladino; Erwin de la Fuente; Alfonso Gonzalez; Chiara Zurzolo |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-10-12 |
Journal Detail:
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Title: Molecular biology of the cell Volume: 22 ISSN: 1939-4586 ISO Abbreviation: Mol. Biol. Cell Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-11-30 Completed Date: 2012-04-12 Revised Date: 2013-05-23 |
Medline Journal Info:
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Nlm Unique ID: 9201390 Medline TA: Mol Biol Cell Country: United States |
Other Details:
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Languages: eng Pagination: 4621-34 Citation Subset: IM |
Affiliation:
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Institut Pasteur, Unité de Traffic Membranaire et Pathogenèse, 75015 Paris, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Polarity Cells, Cultured Cholesterol / metabolism*, pharmacology Dogs Epithelial Cells / cytology, drug effects, metabolism* Fluorescence Recovery After Photobleaching GPI-Linked Proteins / genetics, metabolism* Glycosylation Golgi Apparatus / metabolism, ultrastructure Green Fluorescent Proteins / genetics, metabolism Kidney / cytology Protein Transport Rats Rats, Inbred F344 Recombinant Fusion Proteins / genetics, metabolism Thyroid Gland / cytology, metabolism |
| Chemical | |
Reg. No./Substance:
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0/GPI-Linked Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; 57-88-5/Cholesterol |
| Comments/Corrections | |
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