Document Detail


N-glycans in cell survival and death: cross-talk between glycosyltransferases.
MedLine Citation:
PMID:  22326428     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Asparagine-linked (N-linked) protein glycosylation is one of the most important protein modifications. N-glycans with "high mannose", "hybrid", or "complex" type sugar chains participate in a multitude of cellular processes. These include cell-cell/cell-matrix/receptor-ligand interaction, cell signaling/growth and differentiation, to name a few. Many diseases such as disorders of blood clotting, congenital disorder of glycosylation, diseases of blood vessels, cancer, neo-vascularization, i.e., angiogenesis essential for breast and other solid tumor progression and metastasis are associated with N-glycan expression. Biosynthesis of N-glycans requires multiple steps and multiple cellular compartments. Following transcription and translation the proteins migrate to the endoplasmic reticulum (ER) lumen to acquire glycan chain(s) with a defined glycoform, i.e., a tetradecasaccharide. These are further modified, i.e., edited in ER lumen and in Golgi prior to moving to their respective destinations. The tetradecasaccharide is pre-assembled on a poly-isoprenoid lipid called dolichol, and becomes an essential component of the supply chain. Therefore, dolichol cycle synthesizing the lipid-linked oligosaccharide (LLO) is a hallmark for all N-linked glycoproteins. It is expected that there is a great deal of cross-talk between the participating glycosyltransferases and any missed step would express defective N-glycans that could have fatal consequences. The positive impact of the structurally altered N-glycans could lead to discovery of an N-glycan signature for a disease and/or help developing glycotherapeutic treating cancer or other human diseases. The purpose of this review is to identify the gaps of N-glycan biology and help developing appropriate technology for biomedical applications. This article is part of a Special Issue entitled Glycoproteomics.
Authors:
Dipak K Banerjee
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review     Date:  2012-02-03
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1820     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2012 Sep 
Date Detail:
Created Date:  2012-06-25     Completed Date:  2013-03-07     Revised Date:  2013-04-16    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1338-46     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier B.V. All rights reserved.
Affiliation:
Department of Biochemistry, School of Medicine, University of Puerto Rico, Medical Sciences Campus, San Juan, PR 00936-5067. dipak.banerjee@upr.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Death
Cell Proliferation
Cell Survival
Glycoproteins / biosynthesis,  metabolism*
Glycosyltransferases / metabolism*,  physiology
Humans
Metabolic Networks and Pathways / physiology
Polysaccharides / metabolism*,  physiology*
Grant Support
ID/Acronym/Agency:
U54-CA096297/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Glycoproteins; 0/Polysaccharides; EC 2.4.-/Glycosyltransferases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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