Document Detail


N-glycan biosynthesis inhibitors induce in vitro anticancer activity in colorectal cancer cells.
MedLine Citation:
PMID:  22552949     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
During malignant transformation, changes in the expression profile of glycans may be involved in a variety of events, including the loss of cell-cell and cell-matrix adhesion, migration, invasion, and evasion of apoptosis. Therefore, modulation of glycan expression with drugs has promising therapeutic potential for various cancer types. In this study, we investigated the in vitro anticancer activity of the N-glycan biosynthesis inhibitors (swainsonine and tunicamycin) in cells derived from colorectal cancer. We also examined whether these inhibitors are able to induce radiosensitization and toxicity when used in combination with cisplatin or irinotecan, two current anticancer drugs. Our results show that treatment with tunicamycin inhibits cellular mechanisms related to the malignant phenotype, such as anchorage-dependent and anchorage-independent colony formation, migration and invasion, in undifferentiated HCT-116 colon cancer cells, whereas swainsonine only inhibits cell migration. We also observed that tunicamycin, but not swainsonine, caused radiosensitivity in HCT-116 cells. Moreover, the combination of swainsonine with cisplatin or irinotecan enhanced their toxicity in HCT-116 cells, while the combination of tunicamycin with these drugs had no effect. Given these results, we suggest that the modulation of N-glycan biosynthesis appears to be a potential therapeutic tool for colorectal cancer treatment because inhibition of this process induced anticancer activity in vitro. Additionally, the inhibition of the N-glycan biosynthesis in combination with chemotherapic drugs is a promising therapeutic strategy for enhancing radiation therapy. J. Cell. Biochem. © 2012 Wiley Periodicals, Inc.
Authors:
Julio Cesar Madureira de-Freitas-Junior; Lilian Golçalves Dos Reis Bastos; Carlos Alberto Freire-Neto; Bárbara Du Rocher; Eliana Saul Furquim Werneck Abdelhay; José Andrés Morgado-Díaz
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-5-2
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  -     ISSN:  1097-4644     ISO Abbreviation:  -     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-5-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Wiley Periodicals, Inc.
Affiliation:
Divisão de Biologia Celular, Coordenação de Pesquisa, Instituto Nacional de Câncer, 37André Cavalcanti Street, 5th Floor, Rio de Janeiro, RJ, Brazil, CEP: 20230-051; Programa de Pós-Graduação em Biociências, Universidade do Estado do Rio de Janeiro, 87 Vinte e oito de Setembro Avenue, 4th Floor, Rio de Janeiro, RJ, Brazil, CEP: 20551-030.
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