Document Detail

N-acetylglutamate and N-acetylaspartate in soybeans (Glycine max L.), maize (Zea mays L.), [corrected] and other foodstuffs.
MedLine Citation:
PMID:  18781757     Owner:  NLM     Status:  MEDLINE    
N-Acetylglutamate (NAG) and N-acetylaspartate (NAA) are amino acid derivatives with reported activities in a number of biological processes. However, there is no published information on the presence of either substance in foodstuffs. We developed a method for extracting and quantifying NAG and NAA from soybean seeds and maize grain using ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS). The lower limit of quantification for both NAG and NAA was 1 ng/mL. The method was then utilized to quantify NAG and NAA in other foodstuffs (fruits, vegetables, meats, grains, milk, coffee, tea, cocoa, and others). Both NAG and NAA were present in all of the materials analyzed. The highest concentration of NAG was found in cocoa powder. The highest concentration of NAA was found in roasted coffee beans. Both NAG and NAA were found at quantifiable concentrations in all foods tested indicating that these two acetylated amino acids are common components of the human diet.
Aideen O Hession; Elizabeth G Esrey; Robert A Croes; Carl A Maxwell
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Publication Detail:
Type:  Journal Article     Date:  2008-09-10
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  56     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-10-02     Completed Date:  2008-12-19     Revised Date:  2010-06-24    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  9121-6     Citation Subset:  IM    
Crop Genetics Research and Development, DuPont Agriculture and Nutrition, P.O. Box 80353, Wilmington, Delaware 19880-0353, USA.
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MeSH Terms
Aspartic Acid / analogs & derivatives*,  analysis
Cacao / chemistry
Chromatography, High Pressure Liquid
Coffea / chemistry
Food Analysis*
Glutamates / analysis*
Seeds / chemistry*
Soybeans / chemistry*
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Zea mays / chemistry*
Reg. No./Substance:
0/Glutamates; 1188-37-0/N-acetylglutamate; 56-84-8/Aspartic Acid; 997-55-7/N-acetylaspartate
Erratum In:
J Agric Food Chem. 2009 Oct 28;57(20):9856

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