| N-acetylamino acid utilization by kidney aminoacylase-1. | |
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MedLine Citation:
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PMID: 18222180 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mammalian aminoacylase-1 (Acy1) participates in the breakdown of N-acetylated amino acids during intracellular protein catabolism. Acy1 is most abundantly expressed in the kidney tubular epithelium. Lately, Acy1 deficiency was identified in children with increased urinary excretion of several N-acetylamino acids. Here we report detailed N-acetylamino acid specificity profiles for human and porcine Acy1 based on steady state kinetic measurements. We found that LLC-PK1 cells, a model of the porcine kidney proximal tubular epithelium, robustly express Acy1. For the first time, we demonstrate uptake and utilization of N-acteylleucine and -methionine in replacement of the free amino acid, respectively, in cultured epithelial cells. Our data are consistent with a specific role of kidney Acy1 in the salvage of amino acids originating from systemic degradation of N-acetylated proteins. |
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Authors:
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Holger A Lindner; Margot Täfler-Naumann; Klaus-Heinrich Röhm |
Publication Detail:
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Type: Journal Article Date: 2008-01-05 |
Journal Detail:
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Title: Biochimie Volume: 90 ISSN: 0300-9084 ISO Abbreviation: Biochimie Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-04-28 Completed Date: 2008-08-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1264604 Medline TA: Biochimie Country: France |
Other Details:
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Languages: eng Pagination: 773-80 Citation Subset: IM |
Affiliation:
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Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2, Canada. holger.lindner@cnrc-nrc.gc.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amidohydrolases
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metabolism* Amino Acids / metabolism* Animals Chromatography, High Pressure Liquid Kidney / enzymology* Kinetics LLC-PK1 Cells Rats |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; EC 3.5.-/Amidohydrolases; EC 3.5.1.14/aminoacylase I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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