| N-acetyl-seryl-aspartyl-lysyl-proline prevents cardiac remodeling and dysfunction induced by galectin-3, a mammalian adhesion/growth-regulatory lectin. | |
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MedLine Citation:
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PMID: 19098114 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Galectin-3 (Gal-3) is secreted by activated macrophages. In hypertension, Gal-3 is a marker for hypertrophic hearts prone to develop heart failure. Gal-3 infused in pericardial sac leads to cardiac inflammation, remodeling, and dysfunction. N-acetyl-seryl-aspartyl-lysyl-proline (Ac-SDKP), a naturally occurring tetrapeptide, prevents and reverses inflammation and collagen deposition in the heart in hypertension and heart failure postmyocardial infarction. In the present study, we hypothesize that Ac-SDKP prevents Gal-3-induced cardiac inflammation, remodeling, and dysfunction, and these effects are mediated by the transforming growth factor (TGF)-beta/Smad3 signaling pathway. Adult male rats were divided into four groups and received the following intrapericardial infusion for 4 wk: 1) vehicle (saline, n = 8); 2) Ac-SDKP (800 microg x kg(-1) x day(-1), n = 8); 3) Gal-3 (12 microg/day, n = 7); and 4) Ac-SDKP + Gal-3 (n = 7). Left ventricular ejection fraction, cardiac output, and transmitral velocity were measured by echocardiography; inflammatory cell infiltration, cardiomyocyte hypertrophy, and collagen deposition in the heart by histological and immunohistochemical staining; and TGF-beta expression and Smad3 phosphorylation by Western blot. We found that, in the left ventricle, Gal-3 1) enhanced macrophage and mast cell infiltration, increased cardiac interstitial and perivascular fibrosis, and causes cardiac hypertrophy; 2) increased TGF-beta expression and Smad3 phosphorylation; and 3) decreased negative change in pressure over time response to isoproterenol challenge, ratio of early left ventricular filling phase to atrial contraction phase, and left ventricular ejection fraction. Ac-SDKP partially or completely prevented these effects. We conclude that Ac-SDKP prevents Gal-3-induced cardiac inflammation, fibrosis, hypertrophy, and dysfunction, possibly via inhibition of the TGF-beta/Smad3 signaling pathway. |
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Authors:
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Yun-He Liu; Martin D'Ambrosio; Tang-dong Liao; Hongmei Peng; Nour-Eddine Rhaleb; Umesh Sharma; Sabine André; Hans-J Gabius; Oscar A Carretero |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2008-12-19 |
Journal Detail:
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Title: American journal of physiology. Heart and circulatory physiology Volume: 296 ISSN: 0363-6135 ISO Abbreviation: Am. J. Physiol. Heart Circ. Physiol. Publication Date: 2009 Feb |
Date Detail:
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Created Date: 2009-01-27 Completed Date: 2009-03-12 Revised Date: 2012-10-09 |
Medline Journal Info:
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Nlm Unique ID: 100901228 Medline TA: Am J Physiol Heart Circ Physiol Country: United States |
Other Details:
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Languages: eng Pagination: H404-12 Citation Subset: IM |
Affiliation:
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Hypertension and Vascular Research Division, Department of Internal Medicine, Henry Ford Hospital, Detroit, MI, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Inflammatory Agents / administration & dosage, blood, pharmacology* Blood Pressure / drug effects Body Weight / drug effects Cardiac Output / drug effects Cardiomegaly / chemically induced, metabolism, physiopathology, prevention & control* Cardiotonic Agents / administration & dosage, blood, pharmacology* Collagen / metabolism Disease Models, Animal Echocardiography, Doppler Fibrosis Galectin 3 Heart Rate / drug effects Hemodynamics / drug effects* Inflammation / chemically induced, metabolism, physiopathology, prevention & control* Infusions, Parenteral Isoproterenol / pharmacology Macrophages / drug effects, pathology Male Mast Cells / drug effects, pathology Myocardial Contraction / drug effects Myocardium / metabolism, pathology Oligopeptides / administration & dosage, blood, pharmacology* Phosphorylation Rats Rats, Sprague-Dawley Signal Transduction / drug effects Smad3 Protein / metabolism Stroke Volume / drug effects Time Factors Transforming Growth Factor beta / metabolism Ventricular Function, Left / drug effects* Ventricular Remodeling / drug effects* |
| Grant Support | |
ID/Acronym/Agency:
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HL071806/HL/NHLBI NIH HHS; HL28982/HL/NHLBI NIH HHS; R01 HL071806/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Anti-Inflammatory Agents; 0/Cardiotonic Agents; 0/Galectin 3; 0/Madh3 protein, rat; 0/Oligopeptides; 0/Smad3 Protein; 0/Transforming Growth Factor beta; 120081-14-3/goralatide; 7683-59-2/Isoproterenol; 9007-34-5/Collagen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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