Document Detail

N-acetyl cysteine inhibits cell cycle progression in pancreatic carcinoma cells.
MedLine Citation:
PMID:  11329937     Owner:  NLM     Status:  MEDLINE    
The antioxidant N-acetyl cysteine (NAC) is a precursor of intracellular glutathione (GSH) and is also a well known as one of the chemopreventive agents which act through a variety of cellular mechanisms. We examined the effects of NAC on cell cycle progression in the pancreatic carcinoma cell lines, SW1990 and JHP1. Cells were incubated with or without NAC. Cell cycle distribution was analyzed by flow cytometry and immunoblotting. NAC suppressed cell proliferation in a concentration-dependent manner, whereas NAC increased intracellular glutathione content significantly in a dose-dependent manner. The percentage of cells in the G1 phase after treatment with NAC was significantly higher than the percentage seen for control cells. Cyclin D1 expression of carcinoma cells treated with NAC decreased remarkably compared with cells without NAC treatment. Thus, the antiproliferative effect of NAC by prolongation of the G1 phase in human pancreatic carcinoma cells shows its possible utility as an antitumor agent.
C Kusano; S Takao; H Noma; H Yoh; T Aikou; H Okumura; S Akiyama; M Kawamura; M Makino; M Baba
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Human cell     Volume:  13     ISSN:  0914-7470     ISO Abbreviation:  Hum. Cell     Publication Date:  2000 Dec 
Date Detail:
Created Date:  2001-05-01     Completed Date:  2001-07-12     Revised Date:  2012-05-31    
Medline Journal Info:
Nlm Unique ID:  8912329     Medline TA:  Hum Cell     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  213-20     Citation Subset:  IM    
First Department of Surgery, Faculty of Medicine, Kagoshima University.
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MeSH Terms
Acetylcysteine / pharmacology*
Adenocarcinoma / pathology*
Antineoplastic Agents / pharmacology*
Cell Cycle / drug effects*
Cell Line
Depression, Chemical
Pancreatic Neoplasms / pathology*
Tumor Cells, Cultured
Reg. No./Substance:
0/Antineoplastic Agents; 616-91-1/Acetylcysteine

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