| N-acylethanolamine levels and expression of their metabolizing enzymes during pregnancy. | |
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MedLine Citation:
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PMID: 20534733 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Decidualization is essential for a successful pregnancy and is a tightly regulated process influenced by the local microenvironment. Lipid-based mediators, such as the endocannabinoid anandamide, and other compounds that have cannabimimetic actions may act on the decidua during early pregnancy. In this study, the levels of N-arachidonoylethanolamine (anandamide) and two other N-acylethanolamines, N-oleoylethanolamine and N-palmitoylethanolamine, were measured in rat plasma and maternal tissues between d 8 and 19 of pregnancy by ultraperformance liquid chromatography tandem mass spectrometry. The spatiotemporal expression of N-acylethanolamine metabolizing enzymes in implantation units were also determined by quantitative PCR, Western blot, and immunohistochemistry and shown to vary with gestation being mainly localized in decidual cells. The data also indicated that plasma and tissues levels of all three N-acylethanolamines fluctuate throughout pregnancy. Tissue levels of endocannabinoids did not correlate with plasma, suggesting that during pregnancy, maternal tissue levels of endocannabinoids are primarily regulated by in situ production and degradation to create endocannabinoid gradients conducive to successful pregnancy. |
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Authors:
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B M Fonseca; G Correia-da-Silva; A H Taylor; P M W Lam; T H Marczylo; J C Konje; S C Bell; N A Teixeira |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-09 |
Journal Detail:
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Title: Endocrinology Volume: 151 ISSN: 1945-7170 ISO Abbreviation: Endocrinology Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-27 Completed Date: 2010-09-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
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Languages: eng Pagination: 3965-74 Citation Subset: AIM; IM |
Affiliation:
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Departamento de Bioquímica, Faculdade de Farmácia, and Instituto de Biologia Molecular e Celular, Universidade do Porto, 4099-002 Porto, Portugal. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amidohydrolases
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genetics,
metabolism Animals Cyclooxygenase 2 / genetics, metabolism Decidua / metabolism, physiology Embryo Implantation / genetics, physiology Endocannabinoids / blood, metabolism Enzymes / genetics*, metabolism Ethanolamines / blood*, metabolism* Female Gene Expression Regulation, Enzymologic Gestational Age Phospholipase D / genetics, metabolism Pregnancy / blood*, genetics*, metabolism Rats Rats, Wistar Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Endocannabinoids; 0/Enzymes; 0/Ethanolamines; 0/N-acylethanolamines; EC 1.14.99.1/Cyclooxygenase 2; EC 3.1.4.4/NAPE-PLD protein, rat; EC 3.1.4.4/Phospholipase D; EC 3.5.-/Amidohydrolases; EC 3.5.1.-/fatty-acid amide hydrolase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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