| N-Acetylcysteine Modulates the Cytotoxic Effects of Paclitaxel. | |
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MedLine Citation:
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PMID: 21778718 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: Paclitaxel is a microtubule-stabilizing drug known to cause mitotic G2/M arrest and apoptosis. It also increases the generation of reactive oxygen species (ROS) known to be involved in both apoptotic and necrotic cell death. Antioxidants, such as N-acetylcysteine (NAC), prevent the deleterious effects of ROS and modulate the regulation of apoptotic-linked cellular proteins. Methods: A549 human adenocarcinoma alveolar epithelial cells were treated with 5.0 mM NAC, 1.0 μM paclitaxel, or co-incubated with both NAC and paclitaxel for a 24-hour incubation period. The effects of NAC in paclitaxel-induced cytotoxicity were evaluated by measuring cell viability, production of ROS, and apoptosis. Results: Challenge of cells with paclitaxel resulted in time/concentration-dependent decreases in cell viability and increases in intracellular levels of ROS, and apoptosis, all effects being abrogated by co-treatment with NAC. NAC reduced the paclitaxel-induced increase in activated caspase-10 levels, but potentiated that for caspase-3. Conclusions: NAC alters the cytotoxicity of paclitaxel in vitro by decreasing the levels of ROS, preventing apoptosis, and modulating apoptotic cellular proteins. |
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Authors:
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Patricia Anne Lyle; Panagiotis Mitsopoulos; Zacharias E Suntres |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-20 |
Journal Detail:
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Title: Chemotherapy Volume: 57 ISSN: 1421-9794 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0144731 Medline TA: Chemotherapy Country: - |
Other Details:
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Languages: ENG Pagination: 298-304 Citation Subset: - |
Copyright Information:
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Copyright © 2011 S. Karger AG, Basel. |
Affiliation:
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Medical Sciences Division, Northern Ontario School of Medicine, Thunder Bay, Ont., Canada. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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