| N-Acetyl cysteine restores viability and function of rat odontoblast-like cells impaired by polymethylmethacrylate dental resin extract. | |
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MedLine Citation:
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PMID: 19161674 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There is concern that dental-resin materials directly loaded on a prepared tooth adversely affect dental pulp tissue by releasing the resin chemicals through dentinal tubes. This study determined whether self-curing polymethyl methacrylate (PMMA)-based dental resin extract adversely affected the viability and function of odontoblast-like cells and whether the cytotoxicity of this resin, if any, could be eliminated by N-acetyl cysteine, an antioxidant amino acid derivative. Odontoblast-like cells isolated from rat maxillary incisor dental pulp tissue were exposed to a PMMA resin extract with or without N-acetyl cysteine for 1 h and then cultured in osteoblastic media. The percentage of viable cells 24 h after seeding was 20% in cells exposed to the resin extract without N-acetyl cysteine, whereas 45% of cells were viable after exposure to the N-acetyl cysteine-supplemented extract. The cells that had been exposed to the extract showed a strong tendency for apoptosis associated with the increased reactive oxygen species production and decreased intracellular glutathione level, which was improved by the addition of N-acetyl cysteine. N-Acetyl cysteine supplementation almost completely restored the significantly reduced alkaline phosphatase activity and matrix mineralization by the resin extract. These results conclusively demonstrated that exposure of odontoblast-like cells to the resin extract impaired the cell viability and function and, more intriguingly, N-acetyl cysteine supplementation to the extract significantly prevented these toxic effects. |
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Authors:
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Masahiro Yamada; Norinaga Kojima; Wael Att; Norio Hori; Takeo Suzuki; Takahiro Ogawa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Redox report : communications in free radical research Volume: 14 ISSN: 1743-2928 ISO Abbreviation: Redox Rep. Publication Date: 2009 |
Date Detail:
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Created Date: 2009-01-23 Completed Date: 2009-03-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9511366 Medline TA: Redox Rep Country: England |
Other Details:
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Languages: eng Pagination: 13-22 Citation Subset: IM |
Affiliation:
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The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, California 90095-1668, USA. masa0316@ucla.edu |
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcysteine
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pharmacology* Animals Apoptosis / drug effects Caspase 3 / metabolism Caspase 7 / metabolism Cell Survival / drug effects Cells, Cultured Dental Pulp / cytology Dose-Response Relationship, Drug Flow Cytometry Free Radical Scavengers / pharmacology Glutathione / metabolism Male Membrane Potential, Mitochondrial / drug effects Odontoblasts / cytology, drug effects*, metabolism Polymethyl Methacrylate / toxicity* Rats Rats, Sprague-Dawley Reactive Oxygen Species / metabolism Resins, Synthetic / toxicity* |
| Chemical | |
Reg. No./Substance:
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0/Free Radical Scavengers; 0/Reactive Oxygen Species; 0/Resins, Synthetic; 616-91-1/Acetylcysteine; 70-18-8/Glutathione; 9011-14-7/Polymethyl Methacrylate; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 7 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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