Document Detail


N-Acetyl cysteine restores viability and function of rat odontoblast-like cells impaired by polymethylmethacrylate dental resin extract.
MedLine Citation:
PMID:  19161674     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There is concern that dental-resin materials directly loaded on a prepared tooth adversely affect dental pulp tissue by releasing the resin chemicals through dentinal tubes. This study determined whether self-curing polymethyl methacrylate (PMMA)-based dental resin extract adversely affected the viability and function of odontoblast-like cells and whether the cytotoxicity of this resin, if any, could be eliminated by N-acetyl cysteine, an antioxidant amino acid derivative. Odontoblast-like cells isolated from rat maxillary incisor dental pulp tissue were exposed to a PMMA resin extract with or without N-acetyl cysteine for 1 h and then cultured in osteoblastic media. The percentage of viable cells 24 h after seeding was 20% in cells exposed to the resin extract without N-acetyl cysteine, whereas 45% of cells were viable after exposure to the N-acetyl cysteine-supplemented extract. The cells that had been exposed to the extract showed a strong tendency for apoptosis associated with the increased reactive oxygen species production and decreased intracellular glutathione level, which was improved by the addition of N-acetyl cysteine. N-Acetyl cysteine supplementation almost completely restored the significantly reduced alkaline phosphatase activity and matrix mineralization by the resin extract. These results conclusively demonstrated that exposure of odontoblast-like cells to the resin extract impaired the cell viability and function and, more intriguingly, N-acetyl cysteine supplementation to the extract significantly prevented these toxic effects.
Authors:
Masahiro Yamada; Norinaga Kojima; Wael Att; Norio Hori; Takeo Suzuki; Takahiro Ogawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Redox report : communications in free radical research     Volume:  14     ISSN:  1743-2928     ISO Abbreviation:  Redox Rep.     Publication Date:  2009  
Date Detail:
Created Date:  2009-01-23     Completed Date:  2009-03-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9511366     Medline TA:  Redox Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  13-22     Citation Subset:  IM    
Affiliation:
The Jane and Jerry Weintraub Center for Reconstructive Biotechnology, Division of Advanced Prosthodontics, Biomaterials and Hospital Dentistry, UCLA School of Dentistry, Los Angeles, California 90095-1668, USA. masa0316@ucla.edu
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MeSH Terms
Descriptor/Qualifier:
Acetylcysteine / pharmacology*
Animals
Apoptosis / drug effects
Caspase 3 / metabolism
Caspase 7 / metabolism
Cell Survival / drug effects
Cells, Cultured
Dental Pulp / cytology
Dose-Response Relationship, Drug
Flow Cytometry
Free Radical Scavengers / pharmacology
Glutathione / metabolism
Male
Membrane Potential, Mitochondrial / drug effects
Odontoblasts / cytology,  drug effects*,  metabolism
Polymethyl Methacrylate / toxicity*
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species / metabolism
Resins, Synthetic / toxicity*
Chemical
Reg. No./Substance:
0/Free Radical Scavengers; 0/Reactive Oxygen Species; 0/Resins, Synthetic; 616-91-1/Acetylcysteine; 70-18-8/Glutathione; 9011-14-7/Polymethyl Methacrylate; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspase 7

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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