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The Myxovirus-Resistance Protein, MX1, is a Component of Exosomes Secreted by Uterine Epithelial Cells.
MedLine Citation:
PMID:  22574859     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
PROBLEM: Dairy cattle suffer from high percentages of early embryonic loss, and therefore, it is critical to study the function of the uterus at this time. We hypothesize that the antiviral protein, myxovirus resistance (MX)1, regulates secretion in uterine glandular cells during early pregnancy.
METHOD OF STUDY: Uterine epithelial cells were used to study uterine function, in vitro. Sucrose gradients, Western blotting, and transmission electron microscopy were used to isolate and identify exosomes. Immunofluorescence and ceramide inhibitors were used for the characterization of exosomes.
RESULTS: Myxovirus resistance1 was associated with exosomes and protected from proteases, indicating it was inside exosomes. MX1 partially colocalized with exosomal protein CD63, and a ceramide inhibitor reduced numbers of MX1-associated exosomes.
CONCLUSION: This study is the first to characterize MX1-associated exosomes, and we postulate that MX1 regulates secretion in epithelial cells by playing a role in exosome formation or trafficking.
Authors:
Karen Racicot; Anthony Schmitt; Troy Ott
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  American journal of reproductive immunology (New York, N.Y. : 1989)     Volume:  67     ISSN:  1600-0897     ISO Abbreviation:  Am. J. Reprod. Immunol.     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-05-11     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8912860     Medline TA:  Am J Reprod Immunol     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  498-505     Citation Subset:  IM    
Copyright Information:
© 2012 John Wiley & Sons A/S.
Affiliation:
Department of Obstetric, Gynecology and Reproductive Sciences, School of Medicine, Yale University, New Haven, CT, USA.
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