| Myristoyl-based transport of peptides into living cells. | |
| | |
MedLine Citation:
|
PMID: 18044965 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Translocation of membrane-impermeant molecules to the interior of living cells is a necessity for many biochemical investigations. Myristoylation was studied as a means to introduce peptides into living cells. Uptake of a myristoylated, fluorescent peptide was efficient in the B lymphocyte cell line BA/F3. In contrast, this cell line was resistant to uptake of a cell-penetrating peptide derived from the TAT protein. In BA/F3 cells, membrane association was shown to be rapid, reaching a maximum within 30 min. Cellular uptake of the peptide lagged the membrane association but occurred within a similar time frame. Experiments performed at 37 versus 4 degrees C demonstrated profound temperature dependence in the cellular uptake of myristoylated cargo. Myristoylated peptides with either positive or negative charge were shown to load efficiently. In contrast to TAT-conjugated cargo, pyrenebutyrate did not enhance cellular uptake of the myristoylated peptide. The myristoylated peptide did not adversely affect cell viability at concentrations up to 100 muM. This assessment of myristoyl-based transport provides fundamental data needed in understanding the intracellular delivery of myristoylated peptide cargoes for cell-based biochemical studies. |
| | |
Authors:
|
Allison R Nelson; Laura Borland; Nancy L Allbritton; Christopher E Sims |
Related Documents
:
|
7110505 - Possible role of sialocompounds in the uptake of choline into synaptosomes and nerve ce... 7524505 - Absorption enhancement of hydrophilic compounds by verapamil in caco-2 cell monolayers. 7713875 - Distinguishing between folate receptor-alpha-mediated transport and reduced folate carr... 19748575 - Inhibition of nucleoside transport and synergistic potentiation of methotrexate cytotox... 18435415 - Enrichment of collagen plugs with platelets and amniotic fluid cells increases cell pro... 561715 - Action of a rnase and as rnase on growth of cells in vitro. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural Date: 2007-11-29 |
Journal Detail:
|
Title: Biochemistry Volume: 46 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 2007 Dec |
Date Detail:
|
Created Date: 2007-12-18 Completed Date: 2008-02-27 Revised Date: 2011-09-26 |
Medline Journal Info:
|
Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: United States |
Other Details:
|
Languages: eng Pagination: 14771-81 Citation Subset: IM |
Affiliation:
|
Department of Physiology and Biophysics, University of California, Irvine, California 92697, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Sequence Biological Transport Cell Line, Tumor Disulfides / chemistry, metabolism Gene Products, tat / metabolism Humans Kinetics Lymphocytes / metabolism Molecular Sequence Data Myristic Acid / chemistry* Peptides / chemistry*, metabolism* Static Electricity Temperature |
| Grant Support | |
ID/Acronym/Agency:
|
R01 EB004436-05/EB/NIBIB NIH HHS; R01 EB004597-04/EB/NIBIB NIH HHS; R33 CA105514-04/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Disulfides; 0/Gene Products, tat; 0/Peptides; 544-63-8/Myristic Acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Functional implications for a prototypical K-turn binding protein from structural and dynamical stud...
Next Document: CGFS-type monothiol glutaredoxins from the cyanobacterium Synechocystis PCC6803 and other evolutiona...