Document Detail

Myristic acid increases dense lipoprotein secretion by inhibiting apoB degradation and triglyceride recruitment.
MedLine Citation:
PMID:  12454278     Owner:  NLM     Status:  MEDLINE    
Fatty acids of varying lengths and saturation differentially affect plasma apolipoprotein B-100 (apoB-100) levels. To identify mechanisms at the level of production, rat hepatoma cells, McA-RH7777, were incubated with [(35)S]methionine and either fatty acid-BSA complexes or BSA alone. There were increases in labeled apoB-100 secretion with saturated fatty acids palmitic and myristic (MA) (153 +/- 20% and 165 +/- 11%, respectively, relative to BSA). Incubation with polyunsaturated docosahexaenoic acid (DHA) decreased secretion to 26 +/- 2.0%, while monounsaturated oleic acid (OA) did not change it. In pulse-chase studies, MA treatment resulted in reduced apoB-100 degradation, in agreement with its promotion of secretion. In triglyceride (TG) studies, synthesis was stimulated equally by OA, MA, and DHA, but TG secretion was relatively decreased with MA and DHA. With OA, the majority of newly secreted apoB100-lipoproteins was d < or = 1.006, but with MA, they were much denser (1.063 < d). Furthermore, the relative recruitment of newly synthesized TG to lipoproteins was impaired with MA. We conclude that mechanisms for effects of specific dietary fatty acids on plasma lipoprotein levels may include changes in hepatic production. In turn, hepatic production may be regulated by specific fatty acids at the steps of apoB-100 degradation and the recruitment of nascent TG to lipoprotein particles.
Emma Kummrow; M Mahmood Hussain; Meihui Pan; Julian B Marsh; Edward A Fisher
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of lipid research     Volume:  43     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-27     Completed Date:  2003-06-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2155-63     Citation Subset:  IM    
Department of Biochemistry, Medical College of Pennsylvania-Hahnemann School of Medicine, Philadelphia, PA 19129, USA.
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MeSH Terms
Apolipoproteins B / metabolism*,  secretion
Carcinoma, Hepatocellular
Lipoproteins / metabolism*
Myristic Acid / metabolism*
Triglycerides / metabolism*
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Apolipoproteins B; 0/Lipoproteins; 0/Triglycerides; 544-63-8/Myristic Acid

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