| Mypt1-mediated spatial positioning of Bmp2-producing cells is essential for liver organogenesis. | |
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MedLine Citation:
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PMID: 18776143 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mesodermal tissues produce various inductive signals essential for morphogenesis of endodermal organs. However, little is known about how the spatial relationship between the mesodermal signal-producing cells and their target endodermal organs is established during morphogenesis. Here, we report that a mutation in the zebrafish myosin phosphatase targeting subunit 1 (mypt1) gene causes abnormal bundling of actin filaments and disorganization of lateral plate mesoderm (LPM) and endoderm cells. As a result, the coordination between mesoderm and endoderm cell movements is disrupted. Consequently, the two stripes of Bmp2a-expressing cells in the LPM fail to align in a V-shaped pocket sandwiching the liver primordium. Mispositioning Bmp2a-producing cells with respect to the liver primordium leads to a reduction in hepatoblast proliferation and final abortion of hepatoblasts by apoptosis, causing the liverless phenotype. Our results demonstrate that Mypt1 mediates coordination between mesoderm and endoderm cell movements in order to carefully position the liver primordium such that it receives a Bmp signal that is essential for liver formation in zebrafish. |
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Authors:
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Honghui Huang; Hua Ruan; Meng Yuan Aw; Alamgir Hussain; Lin Guo; Chuan Gao; Feng Qian; Thomas Leung; Haiwei Song; David Kimelman; Zilong Wen; Jinrong Peng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 135 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 2008 Oct |
Date Detail:
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Created Date: 2008-09-08 Completed Date: 2009-01-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 3209-18 Citation Subset: IM |
Affiliation:
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Laboratory of Functional Genomics, Institute of Molecular and Cell Biology, Proteos, Singapore. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Substitution Animals Animals, Genetically Modified Apoptosis Base Sequence Body Patterning / genetics, physiology Bone Morphogenetic Protein 2 / biosynthesis*, genetics Cell Movement Cell Proliferation DNA / genetics Hepatocytes / cytology, metabolism Liver / cytology, embryology*, metabolism* Mesoderm / embryology, metabolism Mutation Myosin-Light-Chain Phosphatase / chemistry, genetics, metabolism* Protein Phosphatase 1 / genetics, metabolism Signal Transduction Zebrafish / embryology*, genetics, metabolism* Zebrafish Proteins / biosynthesis*, genetics |
| Chemical | |
Reg. No./Substance:
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0/Bone Morphogenetic Protein 2; 0/Zebrafish Proteins; 9007-49-2/DNA; EC 3.1.3.16/Protein Phosphatase 1; EC 3.1.3.53/Myosin-Light-Chain Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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