Document Detail

Mypt1-mediated spatial positioning of Bmp2-producing cells is essential for liver organogenesis.
MedLine Citation:
PMID:  18776143     Owner:  NLM     Status:  MEDLINE    
Mesodermal tissues produce various inductive signals essential for morphogenesis of endodermal organs. However, little is known about how the spatial relationship between the mesodermal signal-producing cells and their target endodermal organs is established during morphogenesis. Here, we report that a mutation in the zebrafish myosin phosphatase targeting subunit 1 (mypt1) gene causes abnormal bundling of actin filaments and disorganization of lateral plate mesoderm (LPM) and endoderm cells. As a result, the coordination between mesoderm and endoderm cell movements is disrupted. Consequently, the two stripes of Bmp2a-expressing cells in the LPM fail to align in a V-shaped pocket sandwiching the liver primordium. Mispositioning Bmp2a-producing cells with respect to the liver primordium leads to a reduction in hepatoblast proliferation and final abortion of hepatoblasts by apoptosis, causing the liverless phenotype. Our results demonstrate that Mypt1 mediates coordination between mesoderm and endoderm cell movements in order to carefully position the liver primordium such that it receives a Bmp signal that is essential for liver formation in zebrafish.
Honghui Huang; Hua Ruan; Meng Yuan Aw; Alamgir Hussain; Lin Guo; Chuan Gao; Feng Qian; Thomas Leung; Haiwei Song; David Kimelman; Zilong Wen; Jinrong Peng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  135     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-08     Completed Date:  2009-01-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  3209-18     Citation Subset:  IM    
Laboratory of Functional Genomics, Institute of Molecular and Cell Biology, Proteos, Singapore.
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MeSH Terms
Amino Acid Substitution
Animals, Genetically Modified
Base Sequence
Body Patterning / genetics,  physiology
Bone Morphogenetic Protein 2 / biosynthesis*,  genetics
Cell Movement
Cell Proliferation
DNA / genetics
Hepatocytes / cytology,  metabolism
Liver / cytology,  embryology*,  metabolism*
Mesoderm / embryology,  metabolism
Myosin-Light-Chain Phosphatase / chemistry,  genetics,  metabolism*
Protein Phosphatase 1 / genetics,  metabolism
Signal Transduction
Zebrafish / embryology*,  genetics,  metabolism*
Zebrafish Proteins / biosynthesis*,  genetics
Reg. No./Substance:
0/Bone Morphogenetic Protein 2; 0/Zebrafish Proteins; 9007-49-2/DNA; EC Phosphatase 1; EC Phosphatase

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