Document Detail


Myotoxic activity of an acidic phospholipase A2 isolated from Lachesis muta (Bushmaster) snake venom.
MedLine Citation:
PMID:  10728833     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An acidic phospholipase A2 isolated from Lachesis muta snake venom denoted LM-PLA2, showed neither toxic nor anticoagulant activities in contrast to a potent inhibitory effect of collagen-induced platelet aggregation [Fuly, A.L., Machado. O.L.T., Alves, E.W. and Carlini, C.R., 1997. Thromb. Haemost 78, 1372-1380.]. Now, the myotoxicity induced by LM-PLA2 was investigated by using both in vivo and in vitro experiments. LM-PLA2 induced in vitro a dose- and time-dependent release of creatine-kinase (CK) from mouse Extensor Digitorium Longus (EDL) muscles and also increased the plasma CK activity in treated animals. Histopathological studies confirm myonecrosis of mouse skeletal muscles as a major effect. Edema could also be seen in muscle tissue. The amino-terminal sequence of LM-PLA2 (previously reported) indicates an aspartic acid residue located at position 49, together with other conserved amino acids present in the Asp-49 phospholipases, such as Tyr-28, Gly-30, Gly-32, His-48. Chemical modification of the protein moiety was also performed. Histidine alkylation with p-bromophenacyl bromide and lysine acetylation with acetic anhydride, abolished both indirect hemolytic and myotoxic activities of LM-PLA2. On the other hand, contrarily to what has been observed with several basic myotoxic phospholipases, the myotoxic effect induced by LM-PLA2 was not abolished by heparin.
Authors:
A L Fuly; S Calil-Elias; R B Zingali; J A Guimarães; P A Melo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  38     ISSN:  0041-0101     ISO Abbreviation:  Toxicon     Publication Date:  2000 Jul 
Date Detail:
Created Date:  2000-04-20     Completed Date:  2000-04-20     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  961-72     Citation Subset:  IM    
Affiliation:
Departamento de Bioquímica Médica, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janiero, RJ, Brazil.
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MeSH Terms
Descriptor/Qualifier:
Animals
Creatine Kinase / blood,  metabolism
Mice
Muscles / drug effects*,  pathology
Mycotoxins / chemistry,  isolation & purification,  toxicity*
Phospholipases A / chemistry,  isolation & purification,  toxicity*
Phospholipases A2
Rabbits
Viper Venoms / enzymology*
Viperidae*
Chemical
Reg. No./Substance:
0/Mycotoxins; 0/Viper Venoms; EC 2.7.3.2/Creatine Kinase; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2

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