Document Detail

Myostatin decreases with aerobic exercise and associates with insulin resistance.
MedLine Citation:
PMID:  20386333     Owner:  NLM     Status:  MEDLINE    
PURPOSE: There is mounting evidence that skeletal muscle produces and secretes biologically active proteins or "myokines" that facilitate metabolic cross talk between organ systems. The increased expression of myostatin, a secreted anabolic inhibitor of muscle growth and development, has been associated with obesity and insulin resistance. Despite these intriguing findings, there have been few studies linking myostatin and insulin resistance.
METHODS: To explore this relationship in more detail, we quantified myostatin protein in muscle and plasma from 10 insulin-resistant, middle-aged (53.1 ± 5.5 yr) men before and after 6 months of moderate aerobic exercise training (1200 kcal·wk−¹ at 40%-55% VO2peak). To establish a cause-effect relationship, we also injected C57/Bl6 male mice with high physiological levels of recombinant myostatin protein.
RESULTS: Myostatin protein levels were shown to decrease in muscle (37%, P = 0.042, n = 10) and matching plasma samples (from 28.7 ng·mL−¹ pretraining to 22.8 ng·mL−¹ posttraining, P = 0.003, n = 9) with aerobic exercise. Furthermore, the strong correlation between plasma myostatin levels and insulin sensitivity (R² = 0.82, P < 0.001, n = 9) suggested a cause-effect relationship that was subsequently confirmed by inducing insulin resistance in myostatin-injected mice. A modest increase (44%) in plasma myostatin levels was also associated with significant reductions in the insulin-stimulated phosphorylation of Akt (Thr308) in both muscle and liver of myostatin-treated animals.
CONCLUSIONS: These findings indicate that both muscle and plasma myostatin protein levels are regulated by aerobic exercise and, furthermore, that myostatin is in the causal pathway of acquired insulin resistance with physical inactivity.
Dustin S Hittel; Michelle Axelson; Neha Sarna; Jane Shearer; Kim M Huffman; William E Kraus
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Medicine and science in sports and exercise     Volume:  42     ISSN:  1530-0315     ISO Abbreviation:  Med Sci Sports Exerc     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-22     Completed Date:  2011-02-03     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  8005433     Medline TA:  Med Sci Sports Exerc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2023-9     Citation Subset:  IM; S    
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MeSH Terms
Enzyme-Linked Immunosorbent Assay
Exercise / physiology*
Insulin Resistance / physiology*
Mice, Inbred C57BL
Middle Aged
Muscle, Skeletal / metabolism
Myostatin / blood*
Phosphorylation / physiology
Grant Support
Reg. No./Substance:
0/MSTN protein, human; 0/Mstn protein, mouse; 0/Myostatin

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