Document Detail

Myosin light chain phosphorylation is correlated with cold-induced changes in platelet shape.
MedLine Citation:
PMID:  12126038     Owner:  NLM     Status:  MEDLINE    
Chilling induces shape changes in platelets from disks to spheres with abundant filopodia. Such changes were time-dependent and correlated well with the phosphorylation of 20-kDa myosin light chain (LC20). Both the shape changes and the phosphorylation were reversible. After the platelets had been chilled, myosin became incorporated into the Triton X-insoluble fraction. When the chilled platelets were immunocytochemically stained, anti-myosin antibody was localized with filamentous structures inside the filopodia. These results suggest that LC20 phosphorylation and subsequent interactions with actin filaments play a crucial role in the cold-induced changes in platelet shape and in the formation of filopodia.
H Kawakami; M Higashihara; M Ohsaka; K Miyazaki; M Ikebe; H Hirano
Related Documents :
18432518 - External calcium facilitates signalling, contractile and secretory mechanisms induced a...
15277528 - Rho and rho-kinase mediate thrombin-induced phosphatidylinositol 4-phosphate 5-kinase t...
6130978 - Purification of a troponin i-like factor from pig platelet.
1693228 - Characterization by monoclonal antibody of a highly conserved antigenic determinant exp...
2751648 - Interruption of tpa-induced signals by an antiviral and antitumoral xanthate compound: ...
18393148 - Phenotypic identification of platelet-type von willebrand disease and its discriminatio...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi     Volume:  37     ISSN:  0916-8737     ISO Abbreviation:  J Smooth Muscle Res     Publication Date:  2001 Dec 
Date Detail:
Created Date:  2002-07-19     Completed Date:  2002-07-23     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9211664     Medline TA:  J Smooth Muscle Res     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  113-22     Citation Subset:  IM    
Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Blood Platelets / ultrastructure*
Cold Temperature / adverse effects*
Cytoskeletal Proteins / metabolism
Microscopy, Immunoelectron
Myosin Light Chains / metabolism*
Reg. No./Substance:
0/Cytoskeletal Proteins; 0/Myosin Light Chains

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Principles of office anesthesia: part II. Topical anesthesia.
Next Document:  Smooth muscle changes in varicose veins: an ultrastructural study.