Document Detail


Myosin lever arm directs collective motion on cellular actin network.
MedLine Citation:
PMID:  24591646     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The molecular motor myosin teams up to drive muscle contraction, membrane traffic, and cell division in biological cells. Myosin function in cells emerges from the interaction of multiple motors tethered to a scaffold, with surrounding actin filaments organized into 3D networks. Despite the importance of myosin function, the influence of intermotor interactions on collective motion remains poorly understood. In this study, we used precisely engineered myosin assemblies to examine emergence in collective myosin movement. We report that tethering multiple myosin VI motors, but not myosin V motors, modifies their movement trajectories on keratocyte actin networks. Single myosin V and VI dimers display similar skewed trajectories, albeit in opposite directions, when traversing the keratocyte actin network. In contrast, tethering myosin VI motors, but not myosin V motors, progressively straightens the trajectories with increasing myosin number. Trajectory shape of multimotor scaffolds positively correlates with the stiffness of the myosin lever arm. Swapping the flexible myosin VI lever arm for the relatively rigid myosin V lever increases trajectory skewness, and vice versa. A simplified model of coupled motor movement demonstrates that the differences in flexural rigidity of the two myosin lever arms is sufficient to account for the differences in observed behavior of groups of myosin V and VI motors. In accordance with this model trajectory, shapes for scaffolds containing both myosin V and VI are dominated by the myosin with a stiffer lever arm. Our findings suggest that structural features unique to each myosin type may confer selective advantages in cellular functions.
Authors:
Rizal F Hariadi; Mario Cale; Sivaraj Sivaramakrishnan
Related Documents :
9168866 - The association among occlusal contacts, clenching effort, and bite force distribution ...
23437416 - Amplitude modulation detection by human listeners in reverberant sound fields: carrier ...
23104816 - The attenuation surface for contrast sensitivity has the form of a witch's hat within t...
3461706 - Analysis of moment/force ratios in the mechanics of tooth movement.
23874376 - Correction: difference in adaptive dispersal ability can promote species coexistence in...
20820236 - Temporal averaging of phase measurements in the presence of spurious phase drift: appli...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2014-03-03
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  111     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2014 Mar 
Date Detail:
Created Date:  2014-04-07     Completed Date:  2014-05-29     Revised Date:  2014-09-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4091-6     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Actin Cytoskeleton / metabolism*
Bioengineering / methods
Biomimetics
Biophysics
Models, Biological*
Molecular Dynamics Simulation
Movement / physiology*
Myosins / metabolism*
Nanotechnology / methods
Grant Support
ID/Acronym/Agency:
1DP2CA186752-01/CA/NCI NIH HHS; DP2 CA186752/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
EC 3.6.4.1/Myosins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Individual IKs channels at the surface of mammalian cells contain two KCNE1 accessory subunits.
Next Document:  Molecular interferometer to decode attosecond electron-nuclear dynamics.