Document Detail


Myosin content and myosin isoenzyme distribution in the heterotopic rat heart allograft.
MedLine Citation:
PMID:  2963135     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Heterotopic cardiac transplants are vascularly perfused organs that can be used to study the regulation of myocardial protein content. Prior studies have demonstrated that cardiac isografts undergo marked atrophy with a decrease in weight and myosin content. In the present studies we have investigated the changes in size, myosin content and myosin isoenzyme distribution in the heterotopic cardiac allografts. Six days after transplantation allograft hearts were not spontaneously beating and histologically showed evidence of necrosis and cellular infiltration. Total heart weight (816 +/- 16 mg) and protein content (117 +/- 7 mg) were significantly greater in the allografts compared to in situ hearts (471 +/- 11 and 90 +/- 5 mg respectively, (P less than 0.01). In contrast to the increase in weight there was a simultaneous decrease in myosin ATPase (26%), the V1 isoform of the myosin isoenzyme (43%), and myosin content (53%) in the allograft heart. These studies demonstrate that similar to cardiac isografts, allograft hearts undergo a decrease in myosin content and a shift in myosin isoenzymes. In contrast to the marked atrophy of the cardiac isograft, the allograft heart weight is increased most likely due to rejection with cellular infiltration and an increased water content.
Authors:
I Klein; C Hong; T R Zerbe
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of molecular and cellular cardiology     Volume:  19     ISSN:  0022-2828     ISO Abbreviation:  J. Mol. Cell. Cardiol.     Publication Date:  1987 Sep 
Date Detail:
Created Date:  1988-03-21     Completed Date:  1988-03-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0262322     Medline TA:  J Mol Cell Cardiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  917-21     Citation Subset:  IM    
Affiliation:
Department of Medicine, North Shore University Hospital, Manhasset, New York 11030.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphatases / metabolism*
Animals
Electrophoresis, Polyacrylamide Gel
Heart Transplantation*
Isoenzymes / metabolism*
Myocardium / enzymology*
Myosins / metabolism*
Organ Size
Rats
Grant Support
ID/Acronym/Agency:
5T32 HL07557/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Isoenzymes; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.4.1/Myosins

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