Document Detail


Myosin is a component of the cell surface of cultured cells.
MedLine Citation:
PMID:  4372609     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antisera to myosin from mouse L929 cells, mouse uterus, and rabbit skeletal muscle have been prepared in goats. Each antiserum shows specific reaction by immunodiffusion only to the myosin against which it was prepared. Antiserum against L cell myosin agglutinates L929 cells and shows surface localization in unfixed cells by indirect immunofluorescence. This reaction is prevented by immunoabsorption with L cell myosin, but not by absorption with mouse uterine or rabbit skeletal muscle myosins. Elution of the antibody from L cell myosin antiserum that absorbs to fixed L cells yields antibody reactive to L cell myosin in immunodiffusion. At the ultrastructural level, surface localization is also evident with a peroxidase bridge immunocytologic method as a uniform distribution of surface label. Antiserum against L cell myosin also shows localization on the surface of other cells of rodent origin (3T3-4, NRK, MNRK, KNRK, and MEF) and HeLa cells, but not with some other cells of human origin (ALL and WI-38). The reactivity with antibody of the plasma membrane myosin is unaffected by trypsin treatment or fixation with 0.5-2.0% formalin. These results strongly suggest that myosin in cultured nonmuscle cells is a component of the plasma membrane, a finding of possible significance in studying the mechanisms regulating the motility, morphology, and growth of these cells.
Authors:
M C Willingham; R E Ostlund; I Pastan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  71     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1974 Oct 
Date Detail:
Created Date:  1975-02-06     Completed Date:  1975-02-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  4144-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Agglutination
Animals
Antibodies
Antibody Specificity
Antigen-Antibody Reactions
Cell Line
Cell Membrane / analysis*
Cell Transformation, Neoplastic
Cells, Cultured / ultrastructure*
Female
Fluorescent Antibody Technique
Gammaretrovirus
Goats / immunology
Hela Cells
Histocytochemistry
Humans
Kidney
L Cells (Cell Line)
Leukemia, Lymphoid
Mice
Microscopy, Electron
Moloney murine leukemia virus
Myosins / analysis*,  immunology
Peroxidases
Rats
Chemical
Reg. No./Substance:
0/Antibodies; EC 1.11.1.-/Peroxidases; EC 3.6.4.1/Myosins
Comments/Corrections

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