Document Detail

Myosin-based cortical tension in Dictyostelium resolved into heavy and light chain-regulated components.
MedLine Citation:
PMID:  8793728     Owner:  NLM     Status:  MEDLINE    
Cortical tension in most nonmuscle cells is due largely to force production by conventional myosin (myosin II) assembled into the cytoskeleton. Cytoskeletal contraction in smooth muscle and nonmuscle cells is influenced by the degree of myosin filament assembly, and by activation of myosin motor function via regulatory light chain phosphorylation. Recombinant Dictyostelium discoideum cell lines have been generated bearing altered myosin heavy chains, resulting in either constitutive motor function or constitutive assembly into the cytoskeleton. Analysis of these cells allowed stiffening responses to agonists, measured on single cells, to be resolved into an regulatory light chain-mediated component reflecting activation of motor function, and a myosin heavy chain phosphorylation-regulated component reflecting assembly of filaments into the cytoskeleton. These two components can account for all of the cortical stiffening response seen during tested in vivo contractile events.
T T Egelhoff; T V Naismith; F V Brozovich
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of muscle research and cell motility     Volume:  17     ISSN:  0142-4319     ISO Abbreviation:  J. Muscle Res. Cell. Motil.     Publication Date:  1996 Apr 
Date Detail:
Created Date:  1996-11-04     Completed Date:  1996-11-04     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8006298     Medline TA:  J Muscle Res Cell Motil     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  269-74     Citation Subset:  IM    
Department of Physiology and Biophysics, Case Western Reserve School of Medicine, Cleveland, OH 44106-4970, USA.
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MeSH Terms
Cell Line
Cytoskeleton / physiology*
Dictyostelium / physiology*
Folic Acid
Myosin Heavy Chains / physiology
Myosin Light Chains / physiology
Myosins / physiology*
Grant Support
Reg. No./Substance:
0/Myosin Heavy Chains; 0/Myosin Light Chains; 59-30-3/Folic Acid; EC

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