Document Detail


Myosin VI walks hand-over-hand along actin.
MedLine Citation:
PMID:  15286724     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Myosin VI is a molecular motor that can walk processively on actin filaments with a 36-nm step size. The walking mechanism of myosin VI is controversial because it takes very large steps without an apparent lever arm of required length. Therefore, myosin VI is argued to be the first exception to the widely established lever arm theory. It is therefore critical to directly demonstrate whether this motor walks hand-over-hand along actin despite its short lever arm. Here, we follow the displacement of a single myosin VI head during the stepping process. A single head is displaced 72 nm during stepping, whereas the center of mass previously has been shown to move 36 nm. The most likely explanation for this result is a hand-over-hand walking mechanism. We hypothesize the existence of a flexible element that would allow the motor to bridge the observed 72-nm distance.
Authors:
Zeynep Okten; L Stirling Churchman; Ronald S Rock; James A Spudich
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2004-08-01
Journal Detail:
Title:  Nature structural & molecular biology     Volume:  11     ISSN:  1545-9993     ISO Abbreviation:  Nat. Struct. Mol. Biol.     Publication Date:  2004 Sep 
Date Detail:
Created Date:  2004-08-27     Completed Date:  2004-09-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  101186374     Medline TA:  Nat Struct Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  884-7     Citation Subset:  IM    
Affiliation:
Stanford University School of Medicine, Beckman Center, 279 Campus Drive, Stanford, California 94305-5307, USA.
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MeSH Terms
Descriptor/Qualifier:
Actins / chemistry*
Adenosine Triphosphate / chemistry
Animals
Calmodulin / chemistry
Dose-Response Relationship, Drug
Hydrolysis
Models, Biological
Myosin Heavy Chains / chemistry,  physiology*
Normal Distribution
Plasmids / metabolism
Swine
Time Factors
Grant Support
ID/Acronym/Agency:
GM33289/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Calmodulin; 0/Myosin Heavy Chains; 0/myosin VI; 56-65-5/Adenosine Triphosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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