| Myosin VI has a one track mind versus myosin Va when moving on actin bundles or at an intersection. | |
| | |
MedLine Citation:
|
PMID: 23046080 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Myosin VI (myoVI) and myosin Va (myoVa) serve roles both as intracellular cargo transporters and tethers/anchors. In both capacities, these motors bind to and processively travel along the actin cytoskeleton, a network of intersecting actin filaments and bundles that present directional challenges to these motors. Are myoVI and myoVa inherently different in their abilities to interact and maneuver through the complexities of the actin cytoskeleton? Thus, we created an in vitro model system of intersecting actin filaments and individual unipolar (fascin-actin) or mixed polarity (α-actinin-actin) bundles. The stepping dynamics of individual Qdot-labeled myoVI and myoVa motors were determined on these actin tracks. Interestingly, myoVI prefers to stay on the actin filament it is traveling on, while myoVa switches filaments with higher probability at an intersection or between filaments in a bundle. The structural basis for this maneuverability difference was assessed by expressing a myoVI chimera in which the single myoVI IQ was replaced with the longer, 6 IQ myoVa lever. The mutant behaved more like myoVI at actin intersections and on bundles, suggesting that a structural element other than the lever arm dictates myoVI's preference to stay on track, which may be critical to its role as an intracellular anchor. |
| | |
Authors:
|
M Yusuf Ali; Samantha Beck Previs; Kathleen M Trybus; H Lee Sweeney; David M Warshaw |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-10-9 |
Journal Detail:
|
Title: Traffic (Copenhagen, Denmark) Volume: - ISSN: 1600-0854 ISO Abbreviation: Traffic Publication Date: 2012 Oct |
Date Detail:
|
Created Date: 2012-10-10 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100939340 Medline TA: Traffic Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
© 2012 John Wiley & Sons A/S. |
Affiliation:
|
Department of Molecular Physiology and Biophysics, University of Vermont, Burlington, Vermont, 05405, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Broadband Subwavelength Imaging Using A Tunable Graphene-Lens.
Next Document: Transapical Aortic Valve Implantation: Experiences and Survival Analysis up to Three Years.