Document Detail


Myosin VI has a one track mind versus myosin Va when moving on actin bundles or at an intersection.
MedLine Citation:
PMID:  23046080     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Myosin VI (myoVI) and myosin Va (myoVa) serve roles both as intracellular cargo transporters and tethers/anchors. In both capacities, these motors bind to and processively travel along the actin cytoskeleton, a network of intersecting actin filaments and bundles that present directional challenges to these motors. Are myoVI and myoVa inherently different in their abilities to interact and maneuver through the complexities of the actin cytoskeleton? Thus, we created an in vitro model system of intersecting actin filaments and individual unipolar (fascin-actin) or mixed polarity (α-actinin-actin) bundles. The stepping dynamics of individual Qdot-labeled myoVI and myoVa motors were determined on these actin tracks. Interestingly, myoVI prefers to stay on the actin filament it is traveling on, while myoVa switches filaments with higher probability at an intersection or between filaments in a bundle. The structural basis for this maneuverability difference was assessed by expressing a myoVI chimera in which the single myoVI IQ was replaced with the longer, six IQ myoVa lever. The mutant behaved more like myoVI at actin intersections and on bundles, suggesting that a structural element other than the lever arm dictates myoVI's preference to stay on track, which may be critical to its role as an intracellular anchor.
Authors:
M Yusuf Ali; Samantha B Previs; Kathleen M Trybus; H Lee Sweeney; David M Warshaw
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-30
Journal Detail:
Title:  Traffic (Copenhagen, Denmark)     Volume:  14     ISSN:  1600-0854     ISO Abbreviation:  Traffic     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-01-17     Completed Date:  2013-06-21     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  100939340     Medline TA:  Traffic     Country:  England    
Other Details:
Languages:  eng     Pagination:  70-81     Citation Subset:  IM    
Copyright Information:
© 2012 John Wiley & Sons A/S.
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MeSH Terms
Descriptor/Qualifier:
Actin Cytoskeleton / chemistry,  metabolism*
Actinin / chemistry,  metabolism
Animals
Carrier Proteins / chemistry,  metabolism
Chickens
Mice
Microfilament Proteins / chemistry,  metabolism
Motion
Mutation
Myosin Heavy Chains / chemistry,  genetics,  metabolism*
Myosin Type V / chemistry,  metabolism*
Swine
Grant Support
ID/Acronym/Agency:
DC009100/DC/NIDCD NIH HHS; GM078097/GM/NIGMS NIH HHS; GM094229/GM/NIGMS NIH HHS; R01 DC009100/DC/NIDCD NIH HHS; R01 GM078097/GM/NIGMS NIH HHS; R01 GM094229/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Microfilament Proteins; 0/myosin VI; 11003-00-2/Actinin; 146808-54-0/fascin; EC 3.6.1.-/Myosin Type V; EC 3.6.4.1/Myosin Heavy Chains
Comments/Corrections

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