Document Detail

Myoid cell proliferation in rat seminiferous tubules after ischaemic testicular atrophy induced by epinephrine. Morphometric and immunohistochemical (bromo-deoxyuridine and PCNA) studies.
MedLine Citation:
PMID:  7782129     Owner:  NLM     Status:  MEDLINE    
The proliferation of peritubular myoid cells in the testes of rats treated for 1-11 weeks with intra-scrotal injections of epinephrine was investigated using immunohistochemistry and quantitative histology. The percentage of peritubular cells that were immunopositive for proliferating cell nuclear antigen (PCNA) or that were labelled with 5'-bromodeoxyuridine (BrdU) in the S-phase of the cell cycle, were calculated in control and treated rats after 1,3,5,8 and 11 weeks of treatment. In addition, the change in the number of peritubular cells per testis was calculated using two different stereological methods. The possible correlation between the changes observed using the two proliferation indices (PCNA immunoreaction and labelling of BrdU) in peritubular myoid cells was evaluated by regression analysis. The results of the study indicate that both proliferation indices increased in peritubular cells between the third and the eighth weeks of treatment, and that this increase was correlated with an increase in the number of these cells. From weeks 8-11 of treatment, both proliferation indices decreased and the same occurred with the number of peritubular cells. We hypothesize that proliferation of the peritubular cells occurs in order to increase their secretion of extracellular matrix components leading to enlargement of the lamina propria of the seminiferous tubule.
L Santamaría; R Martín; J Codesal; R Paniagua
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of andrology     Volume:  18     ISSN:  0105-6263     ISO Abbreviation:  Int. J. Androl.     Publication Date:  1995 Feb 
Date Detail:
Created Date:  1995-07-20     Completed Date:  1995-07-20     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8000141     Medline TA:  Int J Androl     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  13-22     Citation Subset:  IM    
Department of Morphology (Histology), School of Medicine, Autonomous University of Madrid, Spain.
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MeSH Terms
Cell Division
Immunoenzyme Techniques
Ischemia / chemically induced,  pathology*
Proliferating Cell Nuclear Antigen / metabolism
Rats, Wistar
Regression Analysis
Seminiferous Tubules / blood supply*,  pathology
Testis / blood supply*,  pathology
Reg. No./Substance:
0/Proliferating Cell Nuclear Antigen; 51-43-4/Epinephrine; 59-14-3/Bromodeoxyuridine

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