Document Detail


Myoglobin's old and new clothes: from molecular structure to function in living cells.
MedLine Citation:
PMID:  20675540     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Myoglobin, a mobile carrier of oxygen, is without a doubt an important player central to the physiological function of heart and skeletal muscle. Recently, researchers have surmounted technical challenges to measure Mb diffusion in the living cell. Their observations have stimulated a discussion about the relative contribution made by Mb-facilitated diffusion to the total oxygen flux. The calculation of the relative contribution, however, depends upon assumptions, the cell model and cell architecture, cell bioenergetics, oxygen supply and demand. The analysis suggests that important differences can be observed whether steady-state or transient conditions are considered. This article reviews the current evidence underlying the evaluation of the biophysical parameters of myoglobin-facilitated oxygen diffusion in cells, specifically the intracellular concentration of myoglobin, the intracellular diffusion coefficient of myoglobin and the intracellular myoglobin oxygen saturation. The review considers the role of myoglobin in oxygen transport in vertebrate heart and skeletal muscle, in the diving seal during apnea as well as the role of the analogous leghemoglobin of plants. The possible role of myoglobin in intracellular fatty acid transport is addressed. Finally, the recent measurements of myoglobin diffusion inside muscle cells are discussed in terms of their implications for cytoarchitecture and microviscosity in these cells and the identification of intracellular impediments to the diffusion of proteins inside cells. The recent experimental data then help to refine our understanding of Mb function and establish a basis for future investigation.
Authors:
Gerolf Gros; Beatrice A Wittenberg; Thomas Jue
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  The Journal of experimental biology     Volume:  213     ISSN:  1477-9145     ISO Abbreviation:  J. Exp. Biol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-02     Completed Date:  2010-11-10     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  0243705     Medline TA:  J Exp Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  2713-25     Citation Subset:  IM    
Affiliation:
Zentrum Physiologie, Medizinische Hochschule Hannover, 30625 Hannover, Germany.
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MeSH Terms
Descriptor/Qualifier:
Animals
Diffusion
Diving / physiology
Fatty Acids / metabolism
Humans
Muscle, Skeletal / metabolism,  ultrastructure
Myocardium / metabolism
Myoglobin / chemistry*,  genetics,  metabolism*
Nuclear Magnetic Resonance, Biomolecular
Oxygen / metabolism
Oxygen Consumption / physiology
Grant Support
ID/Acronym/Agency:
GM 58688/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Myoglobin; 7782-44-7/Oxygen
Comments/Corrections

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