Document Detail


Myogenic tone of the rat testicular subcapsular artery has a role in autoregulation of testicular blood supply.
MedLine Citation:
PMID:  2346776     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An in vitro method is described for the isolation, cannulation, and pressurization of a 10-mm segment of the adult rat testicular subcapsular artery by use of a 200-microns micropipette connected to an adjustable-height reservoir. External and internal arterial diameters were measured by a digital filar micrometer eyepiece calibrated with a Microcode scaler. Transmural pressure was increased stepwise by 20 mm Hg from 20 to 180 mm Hg at 10-min intervals. The following novel triphasic myogenic response was found: a 22.5% increase in lumen cross-sectional area from 20 to 40 mm Hg (p less than 0.05); a 37.3% decrease in lumen cross-sectional area from 40 to 100 mm Hg (p less than 0.05); and a 72.4% increase in lumen cross-sectional area from 100 to 180 mm Hg (p less than 0.05). Papaverine (0.1 mM) completely blocked the transmural pressure-induced vasoconstriction, indicating an active increase in vascular smooth muscle tone by transmural pressure. The calculated blood flow through the testicular subcapsular artery of the adult rat, from measured internal radii at various transmural pressures ranging from 20 to 180 mm Hg by Poiseuille's equation, was constant from 40 to 100 mm Hg and showed a progressive increase from 100 to 180 mm Hg. These data suggest that the myogenic response of the adult rat testicular subcapsular artery may have an important role in the autoregulation of the testicular blood supply.
Authors:
J R Davis
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Biology of reproduction     Volume:  42     ISSN:  0006-3363     ISO Abbreviation:  Biol. Reprod.     Publication Date:  1990 Apr 
Date Detail:
Created Date:  1990-07-11     Completed Date:  1990-07-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  727-35     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, Loyola University of Chicago, Stritch School of Medicine, Maywood, Illinois 60153.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arteries / drug effects,  physiology
Blood Pressure / physiology
Catheterization / methods
Culture Techniques / methods
Homeostasis / physiology*
Male
Muscle Tonus
Papaverine / pharmacology
Rats
Rats, Inbred Strains
Regional Blood Flow
Testis / blood supply*
Vasodilation
Chemical
Reg. No./Substance:
58-74-2/Papaverine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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