| Myogenic skeletal muscle satellite cells communicate by tunnelling nanotubes. | |
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MedLine Citation:
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PMID: 20112291 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Quiescent satellite cells sit on the surface of the muscle fibres under the basal lamina and are activated by a variety of stimuli to disengage, divide and differentiate into myoblasts that can regenerate or repair muscle fibres. Satellite cells adopt their parent's fibre type and must have some means of communication with the parent fibre. The mechanisms behind this communication are not known. We show here that satellite cells form dynamic connections with muscle fibres and other satellite cells by F-actin based tunnelling nanotubes (TNTs). Our results show that TNTs readily develop between satellite cells and muscle fibres. Once developed, TNTs permit transport of intracellular material, and even cellular organelles such as mitochondria between the muscle fibre and satellite cells. The onset of satellite cell differentiation markers Pax-7 and MyoD expression was slower in satellite cells cultured in the absence than in the presence of muscle cells. Furthermore physical contact between myofibre and satellite cell progeny is required to maintain subtype identity. Our data establish that TNTs constitute an integral part of myogenic cell communication and that physical cellular interaction control myogenic cell fate determination. |
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Authors:
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Pasi Tavi; Topi Korhonen; Sandra L H?nninen; Joseph D Bruton; Sara L??f; Andras Simon; H?kan Westerblad |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of cellular physiology Volume: 223 ISSN: 1097-4652 ISO Abbreviation: J. Cell. Physiol. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-03-01 Completed Date: 2010-05-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 376-83 Citation Subset: IM |
Affiliation:
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Department of Biotechnology and Molecular Medicine, A. I. Virtanen Institute for Molecular Medicine, University of Kuopio, FIN-70211 Kuopio, Finland. pasi.tavi@uku.fi |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Transport, Active / physiology Cell Communication / physiology* Cell Differentiation / physiology Cell Lineage / physiology Cell Surface Extensions / metabolism, ultrastructure Cells, Cultured Culture Media, Conditioned / pharmacology Male Mice Microfilaments / metabolism, ultrastructure Muscle Development / physiology* Muscle Fibers, Skeletal / metabolism, ultrastructure Muscle, Skeletal / cytology, growth & development*, metabolism* MyoD Protein / metabolism Nanotubes, Peptide* PAX7 Transcription Factor / metabolism Phenotype Satellite Cells, Skeletal Muscle / physiology*, ultrastructure |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/MyoD Protein; 0/MyoD1 myogenic differentiation protein; 0/Nanotubes, Peptide; 0/PAX7 Transcription Factor; 0/Pax7 protein, mouse |
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