| Myogenic and proteolytic mRNA expression following blood flow restricted exercise. | |
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MedLine Citation:
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PMID: 20653608 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: Resistance exercise performed at low loads (20-30% of maximal strength) with blood flow restriction (BFR) acutely increases protein synthesis and induces hypertrophy when performed chronically. We investigated myogenic and proteolytic mRNA expression 8 h following an acute bout of knee extension exercise. METHODS: Fifteen subjects (22.8 ± 3.7 years, eight men and seven women) were randomized to two exercise conditions: BFR or control exercise. All participants performed four sets of exercise (30, 15, 15 and 15 repetitions) at 20% of maximal strength. Persons in the BFR group had a cuff placed on the upper thigh inflated to 1.5 times brachial systolic blood pressure (cuff pressure range: 135-186 mmHg). Muscle biopsies from the vastus lateralis were excised 24 h before and 8 h following the exercise. Results: RT-PCR analysis demonstrated no change in myogenic gene expression (insulin-like growth factor-1, MyoD, myogenin, myostatin - a negative regulator) with either exercise condition (P > 0.123). However, BFR exercise downregulated mRNA expression in transcripts associated with proteolytic pathways (FOXO3A, Atrogin-1 and MuRF-1) with no change in the control exercise condition. Specifically, median mRNA expression of FOXO3A decreased by 1.92-fold (P = 0.01), Atrogin-1 by 2.10-fold (P = 0.01) and MuRF-1 by 2.44-fold (P = 0.01). Conclusion: These data are consistent with the downregulation of proteolytic transcripts observed following high-load resistance exercise. In summary, myogenic genes are unchanged and proteolytic genes associated with muscle remodelling are reduced 8 h following low-load BFR exercise. |
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Authors:
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T M Manini; K R Vincent; C L Leeuwenburgh; H A Lees; A N Kavazis; S E Borst; B C Clark |
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Publication Detail:
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Type: Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Acta physiologica (Oxford, England) Volume: 201 ISSN: 1748-1716 ISO Abbreviation: Acta Physiol (Oxf) Publication Date: 2011 Feb |
Date Detail:
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Created Date: 2011-01-18 Completed Date: 2011-05-04 Revised Date: 2012-02-02 |
Medline Journal Info:
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Nlm Unique ID: 101262545 Medline TA: Acta Physiol (Oxf) Country: England |
Other Details:
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Languages: eng Pagination: 255-63 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Acta Physiologica © 2010 Scandinavian Physiological Society. |
Affiliation:
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Department of Aging and Geriatric Research, University of Florida, Gainesville, FL 32611-0107, USA. tmanini@aging.ufl.edu |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Electromyography Female Humans Hypertrophy Male Muscle Development Muscle Proteins / biosynthesis* Quadriceps Muscle / blood supply, growth & development, metabolism* RNA, Messenger / metabolism Regional Blood Flow Resistance Training* Young Adult |
| Grant Support | |
ID/Acronym/Agency:
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P30 AG021332-01/AG/NIA NIH HHS; P30AG028740/AG/NIA NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Muscle Proteins; 0/RNA, Messenger |
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