Document Detail

Myogenic and proteolytic mRNA expression following blood flow restricted exercise.
MedLine Citation:
PMID:  20653608     Owner:  NLM     Status:  MEDLINE    
AIM: Resistance exercise performed at low loads (20-30% of maximal strength) with blood flow restriction (BFR) acutely increases protein synthesis and induces hypertrophy when performed chronically. We investigated myogenic and proteolytic mRNA expression 8 h following an acute bout of knee extension exercise.
METHODS: Fifteen subjects (22.8 ± 3.7 years, eight men and seven women) were randomized to two exercise conditions: BFR or control exercise. All participants performed four sets of exercise (30, 15, 15 and 15 repetitions) at 20% of maximal strength. Persons in the BFR group had a cuff placed on the upper thigh inflated to 1.5 times brachial systolic blood pressure (cuff pressure range: 135-186 mmHg). Muscle biopsies from the vastus lateralis were excised 24 h before and 8 h following the exercise.
RESULTS:   RT-PCR analysis demonstrated no change in myogenic gene expression (insulin-like growth factor-1, MyoD, myogenin, myostatin - a negative regulator) with either exercise condition (P > 0.123). However, BFR exercise downregulated mRNA expression in transcripts associated with proteolytic pathways (FOXO3A, Atrogin-1 and MuRF-1) with no change in the control exercise condition. Specifically, median mRNA expression of FOXO3A decreased by 1.92-fold (P = 0.01), Atrogin-1 by 2.10-fold (P = 0.01) and MuRF-1 by 2.44-fold (P = 0.01).
CONCLUSION:   These data are consistent with the downregulation of proteolytic transcripts observed following high-load resistance exercise. In summary, myogenic genes are unchanged and proteolytic genes associated with muscle remodelling are reduced 8 h following low-load BFR exercise.
T M Manini; K R Vincent; C L Leeuwenburgh; H A Lees; A N Kavazis; S E Borst; B C Clark
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Acta physiologica (Oxford, England)     Volume:  201     ISSN:  1748-1716     ISO Abbreviation:  Acta Physiol (Oxf)     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-18     Completed Date:  2011-05-04     Revised Date:  2014-07-30    
Medline Journal Info:
Nlm Unique ID:  101262545     Medline TA:  Acta Physiol (Oxf)     Country:  England    
Other Details:
Languages:  eng     Pagination:  255-63     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. Acta Physiologica © 2010 Scandinavian Physiological Society.
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MeSH Terms
Muscle Development
Muscle Proteins / biosynthesis*
Quadriceps Muscle / blood supply,  growth & development,  metabolism*
RNA, Messenger / metabolism
Regional Blood Flow
Resistance Training*
Young Adult
Grant Support
P30 AG021332/AG/NIA NIH HHS; P30 AG021332-01/AG/NIA NIH HHS; P30AG028740/AG/NIA NIH HHS
Reg. No./Substance:
0/Muscle Proteins; 0/RNA, Messenger

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