Document Detail

Myogenic differentiation of Drosophila Schneider cells by DNA double-strand break-inducing drugs.
MedLine Citation:
PMID:  12823228     Owner:  NLM     Status:  MEDLINE    
Drosophila melanogaster has been widely used as a model organism to study various aspects of development. Apart from the whole Drosophila embryo, there are a number of cultured cell lines derived from Drosophila embryo that have also been used for elucidating various aspects of development. Drosophila Schneider line 2 cells were derived from the late stages of the embryo (Schneider, 1972). We found that the Schneider cells undergo myogenic differentiation upon treatment with neocarzinostatin (NCS), DNA double-strand break (DSB)-inducing drug, as indicated by elongated morphology, myosin heavy chain protein expression, multinucleation and exit from the cell cycle. No induction of differentiation was observed when cell proliferation was inhibited with drugs that do not cause DNA DSBs. Pre-treatment of Schneider cells with inhibitors of PKC, PP 1/2A, p38 MAPK, JNK and proteasomes resulted in the inhibition of morphological differentiation induced by NCS. These results indicate that DNA DSBs can turn on the myogenic program in Drosophila Schneider cells and the process is dependent on PK C-, PP 1/2A-, p38 MAPK-, and JNK- mediated signaling and proteasomal activity. The molting hormone, 20-hydroxyecdysone (20-HE), also showed an anti-myogenic effect on the process. This is the first report of insect cells undergoing differentiation by DNA DSB-inducing drugs as far as we know, and it provides a very useful and convenient in vitro system to study various aspects of Drosophila myogenesis.
Muktadir S Hossain; Nobuyoshi Akimitsu; Kenji Kurokawa; Kazuhisa Sekimizu
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Differentiation; research in biological diversity     Volume:  71     ISSN:  0301-4681     ISO Abbreviation:  Differentiation     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-06-25     Completed Date:  2004-03-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401650     Medline TA:  Differentiation     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  271-80     Citation Subset:  IM    
Laboratory of Developmental Biochemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, 3-1, 7-Chome, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
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MeSH Terms
Acetylcysteine / analogs & derivatives*,  pharmacology
Cell Differentiation / drug effects*
Cysteine Proteinase Inhibitors / pharmacology
DNA Damage / drug effects*
Drosophila / drug effects
Ecdysterone / pharmacology
Fluorescent Dyes
Microscopy, Phase-Contrast
Muscle Cells / drug effects*
Myosin Heavy Chains / biosynthesis,  genetics
Nucleic Acid Synthesis Inhibitors / pharmacology*
Oxidative Stress / physiology
Signal Transduction / physiology
Zinostatin / pharmacology*
Reg. No./Substance:
0/Cysteine Proteinase Inhibitors; 0/Fluorescent Dyes; 0/Indoles; 0/Myosin Heavy Chains; 0/Nucleic Acid Synthesis Inhibitors; 133343-34-7/lactacystin; 47165-04-8/DAPI; 5289-74-7/Ecdysterone; 616-91-1/Acetylcysteine; 9014-02-2/Zinostatin

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