| Myogenic capacity of muscle progenitor cells from head and limb muscles. | |
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MedLine Citation:
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PMID: 22288919 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Grefte S, Kuijpers MAR, Kuijpers-Jagtman AM, Torensma R, Von den Hoff JW. Myogenic capacity of muscle progenitor cells from head and limb muscles. Eur J Oral Sci 2012; 120: 38-45. © 2012 Eur J Oral Sci The restoration of muscles in the soft palate of patients with cleft lip and/or palate is accompanied by fibrosis, which leads to speech and feeding problems. Treatment strategies that improve muscle regeneration have only been tested in limb muscles. Therefore, in the present study the myogenic potential of muscle progenitor cells (MPCs) isolated from head muscles was compared with that of limb muscles. Muscle progenitor cells were isolated from the head muscles and limb muscles of rats and cultured. The proliferation of MPCs was analysed by DNA quantification. The differentiation capacity was analysed by quantifying the numbers of fused cells, and by measuring the mRNA levels of differentiation markers. Muscle progenitor cells were stained to quantify the expression of paired box protein Pax 7 (Pax-7), myoblast determination protein 1 (MyoD), and myogenin. Proliferation was similar in the head MPCs and the limb MPCs. Differentiating head and limb MPCs showed a comparable number of fused cells and mRNA expression levels of myosin-1 (Myh1), myosin-3 (Myh3), and myosin-4 (Myh4). During proliferation and differentiation, the number of Pax-7(+) , MyoD(+) , and myogenin(+) cells in head and limb MPCs was equal. It was concluded that head and limb MPCs show similar myogenic capacities in vitro. Therefore, in vivo myogenic differences between those muscles might rely on the local microenvironment. Thus, regenerative strategies for limb muscles might also be used for head muscles. |
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Authors:
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Sander Grefte; Mette A R Kuijpers; Anne M Kuijpers-Jagtman; Ruurd Torensma; Johannes W Von den Hoff |
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Publication Detail:
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Type: Journal Article Date: 2012-01-23 |
Journal Detail:
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Title: European journal of oral sciences Volume: 120 ISSN: 1600-0722 ISO Abbreviation: Eur. J. Oral Sci. Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9504563 Medline TA: Eur J Oral Sci Country: Denmark |
Other Details:
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Languages: eng Pagination: 38-45 Citation Subset: D; IM |
Copyright Information:
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© 2012 Eur J Oral Sci. |
Affiliation:
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Radboud University Nijmegen Medical Centre, Department of Orthodontics and Craniofacial Biology, Nijmegen Radboud University Nijmegen Medical Centre, Tumor Immunology, Nijmegen, the Netherlands. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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