| Myocardial self-preservative effect of heat shock protein 70 on an immature lamb heart. | |
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MedLine Citation:
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PMID: 10585051 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Heat shock proteins have been shown to enhance myocardial tolerance of ischemia-reperfusion injury and are induced in the myocardium of many animals by various stressors. METHODS: To assess the effects and time course of the inducible form of heat shock protein 70, we raised the rectal temperature of 15 neonatal lambs to 43 degrees C for 15 minutes. At 15, 30, 60, and 120 minutes and 24 hours after heat shock, hearts were subjected to immunoblot analysis for heat shock protein (hsp 72/73). Twenty-four hours after heat shock, neonatal lamb hearts (n = 8) were subjected to 2 hours of cold cardioplegic ischemia (HSP group). Eight neonatal lamb hearts without heat shock served as control. After 60 minutes of reperfusion, left ventricular systolic and diastolic function, coronary blood flow (CBF), myocardial oxygen consumption (MVO2), and lactate levels were measured. Endothelial function was assessed by measuring in situ coronary vascular resistance response to acetylcholine and trinitroglycerine. RESULTS: The HSP group showed a significantly higher recovery of systolic function as well as MVO, and a lower lactate level compared to the control group at 60 minutes after reperfusion. Recovery of coronary endothelial function was also significantly better in the HSP group than in the control group. Inducible form of HSP 70 was expressed 15 minutes after heat shock and continued to be observed at 24 hours after the stress. CONCLUSIONS: Heat shock stress associated with the production of inducible heat shock proteins improved the recovery of ventricular function as well as endothelial function and aerobic metabolism after hypothermic cardioplegic ischemia. Induction of heat shock proteins by any means prior to planned hypothermic ischemia may lead to a new approach for myocardial protection. |
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Authors:
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F Nomura; M Aoki; J M Forbess; J E Mayer |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Annals of thoracic surgery Volume: 68 ISSN: 0003-4975 ISO Abbreviation: Ann. Thorac. Surg. Publication Date: 1999 Nov |
Date Detail:
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Created Date: 1999-12-14 Completed Date: 1999-12-14 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 15030100R Medline TA: Ann Thorac Surg Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1736-41 Citation Subset: AIM; IM |
Affiliation:
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Department of Cardiovascular Surgery, Children's Hospital, Boston, Massachusetts, USA. fnomura@kure-nh.go.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Coronary Circulation / physiology Endothelium, Vascular / physiopathology HSP70 Heat-Shock Proteins / physiology* Ischemic Preconditioning, Myocardial Lactic Acid / blood Myocardial Reperfusion Injury / pathology, physiopathology* Myocardium / pathology* Oxygen Consumption / physiology Sheep Vascular Resistance / physiology Ventricular Function, Left / physiology |
| Chemical | |
Reg. No./Substance:
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0/HSP70 Heat-Shock Proteins; 50-21-5/Lactic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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