Document Detail


Myocardial infarction and intramyocardial injection models in swine.
MedLine Citation:
PMID:  22790084     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sustainable and reproducible large animal models that closely replicate the clinical sequelae of myocardial infarction (MI) are important for the translation of basic science research into bedside medicine. Swine are well accepted by the scientific community for cardiovascular research, and they represent an established animal model for preclinical trials for US Food and Drug Administration (FDA) approval of novel therapies. Here we present a protocol for using porcine models of MI created with a closed-chest coronary artery occlusion-reperfusion technique. This creates a model of MI encompassing the anteroapical, lateral and septal walls of the left ventricle. This model infarction can be easily adapted to suit individual study design and enables the investigation of a variety of possible interventions. This model is therefore a useful tool for translational research into the pathophysiology of ventricular remodeling and is an ideal testing platform for novel biological approaches targeting regenerative medicine. This model can be created in approximately 8-10 h.
Authors:
Frederic C McCall; Kartik S Telukuntla; Vasileios Karantalis; Viky Y Suncion; Alan W Heldman; Muzammil Mushtaq; Adam R Williams; Joshua M Hare
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-07-12
Journal Detail:
Title:  Nature protocols     Volume:  7     ISSN:  1750-2799     ISO Abbreviation:  Nat Protoc     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-07-13     Completed Date:  2012-11-30     Revised Date:  2014-03-11    
Medline Journal Info:
Nlm Unique ID:  101284307     Medline TA:  Nat Protoc     Country:  England    
Other Details:
Languages:  eng     Pagination:  1479-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Coronary Occlusion
Coronary Vessels
Disease Models, Animal*
Heart Ventricles / physiopathology
Injections
Myocardial Infarction / physiopathology*
Myocardial Reperfusion Injury / physiopathology
Stem Cells
Swine
Time Factors
Grant Support
ID/Acronym/Agency:
P20 HL101443/HL/NHLBI NIH HHS; P20 HL101443/HL/NHLBI NIH HHS; R01 HL084275/HL/NHLBI NIH HHS; R01 HL084275/HL/NHLBI NIH HHS; R01 HL094849/HL/NHLBI NIH HHS; R01 HL094849/HL/NHLBI NIH HHS; R01 HL107110/HL/NHLBI NIH HHS; R01 HL107110/HL/NHLBI NIH HHS; R01 HL110737/HL/NHLBI NIH HHS; R01 HL110737/HL/NHLBI NIH HHS; U54 HL081028/HL/NHLBI NIH HHS; U54 HL081028/HL/NHLBI NIH HHS; UM1 HL113460/HL/NHLBI NIH HHS
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