Document Detail


Myocardial and circulating levels of microRNA-21 reflect left ventricular fibrosis in aortic stenosis patients.
MedLine Citation:
PMID:  22882958     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Various human cardiovascular pathophysiological conditions associate aberrant expression of microRNAs (miRNAs) and circulating miRNAs are emerging as promising biomarkers. In mice, myocardial miR-21 overexpression is related to cardiac fibrosis elicited by pressure overload. This study was designed to determine the role of myocardial and plasmatic miR-21 in the maladaptive remodeling of the extracellular matrix induced by pressure overload in aortic stenosis (AS) patients and the clinical value of miR-21 as a biomarker for pathological myocardial fibrosis. METHODS: In left ventricular biopsies from 75 AS patients and 32 surgical controls, we quantified the myocardial transcript levels of miR-21, miR-21-targets and ECM- and TGF-β-signaling-related elements. miR-21 plasma levels were determined in 25 healthy volunteers and in AS patients. In situ hybridization of miR-21 was performed in myocardial sections. RESULTS: The myocardial and plasma levels of miR-21 were significantly higher in the AS patients compared with the controls and correlated directly with the echocardiographic mean transvalvular gradients. miR-21 overexpression was confined to interstitial cells and absent in cardiomyocytes. Using bootstrap validated multiple linear regression, the variance in myocardial collagen expression was predicted by myocardial miR-21 (70% of collagen variance) or plasma miR-21 (52% of collagen variance), together with the miR-21 targets RECK and PDCD4, and effectors of TGF-ß signaling. CONCLUSIONS: Our results support the role of miR-21 as a regulator of the fibrotic process that occurs in response to pressure overload in AS patients and underscore the value of circulating miR-21 as a biomarker for myocardial fibrosis.
Authors:
Ana V Villar; Raquel García; David Merino; Miguel Llano; Manuel Cobo; Cecilia Montalvo; Rafael Martín-Durán; María A Hurlé; J Francisco Nistal
Related Documents :
22592898 - Reduced endoglin activity limits cardiac fibrosis and improves survival in heart failure.
7277518 - Return to work after myocardial infarction in a lower socioeconomic population.
22876298 - Scn5a mutations in brugada syndrome are associated with increased cardiac dimensions an...
22177908 - Coronary flow in neonates with impaired intrauterine growth.
7786668 - Reversal of haemochromatotic cardiomyopathy in beta thalassaemia by chelation therapy.
16002158 - Inaccuracy of dipyridamole echocardiography or scintigraphy for the diagnosis of corona...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-8
Journal Detail:
Title:  International journal of cardiology     Volume:  -     ISSN:  1874-1754     ISO Abbreviation:  Int. J. Cardiol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8200291     Medline TA:  Int J Cardiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
Affiliation:
Universidad de Cantabria, Santander, Spain; Instituto de Formación e Investigación Marqués de Valdecilla (IFIMAV), Santander, Spain.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Morphological and physicochemical characteristics of iron corrosion scales formed under different wa...
Next Document:  Impact of epicardial fat volume on coronary artery disease in symptomatic patients with a zero calci...