| Myocardial calcium-independent phospholipase A2 activity during global ischemia in isolated rabbit hearts. | |
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MedLine Citation:
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PMID: 8759249 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVES: To study calcium-independent phospholipase A2 activity during global ischemia in isolated rabbit hearts by measuring the hydrolysis of the endogenous choline phospholipids. METHODS: Langendorff perfused rabbit hearts were exposed to global ischemia for 15 or 60 min, or control perfusion for the same length of time. The hearts were then rapidly frozen in liquid nitrogen and lyophilized. Calcium-independent phospholipase A2 activity in the lyophilized tissue was studied by measuring accumulation of lysophospholipids resulting from hydrolysis of both the choline diacylphospholipid and the choline plasmalogen pool. RESULTS: The calcium-independent phospholipase A2 activity showed the same pH, temperature and calcium sensitivity in control and ischemic (15 min of ischemia) lyophilized myocardial tissue. Incubation of control and ischemic tissue showed no difference in the rate of accumulation of lysophospholipids when the ischemic tissue was obtained from hearts exposed to 15 min of ischemia (107 +/- 4 vs 111 +/- 7 nmol/g dry wt x min, ischemia versus control, mean +/- s.e.m., n = 8), but a significant decrease was noticed in tissue from hearts that had been exposed to 60 min of ischemia (31 +/- 9 vs 86 +/- 18 nmol/g dry wt x min, P < 0.05, n = 4). The decreased phospholipase A2 activity in tissue exposed to 60 min of ischemia was not due to enhanced metabolism of the lysophospholipids (84 +/- 15 vs 79 +/- 8 nmol/g dry wt x min, n = 4). The calcium-independent phospholipase A2 activity was considerably lower in fresh myocardial tissue compared with lyophilized tissue, but comparison of control and ischemic fresh tissue gave results comparable to those found using lyophilized tissue. The myocardial calcium-independent phospholipase A2 activity showed no plasmalogen selectivity in either control or ischemic myocardium. CONCLUSIONS: In isolated perfused rabbit hearts we found no evidence for activation of calcium-independent phospholipase A2 activity during global ischemia. With prolonged time of ischemia there was a significant decrease in calcium-independent phospholipase A2 activity. |
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Authors:
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O Vesterqvist; C A Sargent; G J Grover; M L Ogletree |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cardiovascular research Volume: 31 ISSN: 0008-6363 ISO Abbreviation: Cardiovasc. Res. Publication Date: 1996 Jun |
Date Detail:
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Created Date: 1996-10-11 Completed Date: 1996-10-11 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0077427 Medline TA: Cardiovasc Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 932-40 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Bristol-Myers Squibb Pharmaceutical Research Insititute, Princeton, NJ 08543-4000, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism Chromatography, High Pressure Liquid Female Lysophospholipids / metabolism Myocardial Ischemia / enzymology* Myocardium / enzymology*, metabolism Perfusion Phospholipases A / analysis, metabolism* Phospholipases A2 Rabbits Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Lysophospholipids; 7440-70-2/Calcium; EC 3.1.1.-/Phospholipases A; EC 3.1.1.4/Phospholipases A2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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