Document Detail


Myoblast differentiation during mammalian somitogenesis is dependent upon a community effect.
MedLine Citation:
PMID:  7892257     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The differentiation potential of early mammalian myogenic cells was tested under clonal culture conditions. Cells were isolated from paraxial mesoderm and limb buds of transgenic mouse embryos at 9.5 days after conception and grown in culture at clonal density either on collagen-coated dishes or on various feeder cell layers. The transgene used contained a reporter gene encoding beta-galactosidase with a nuclear localization signal under the control of regulatory sequences from the gene for fast myosin light chain 3, so that beta-galactosidase staining indicated the presence of differentiated muscle cells. After 5 days in culture, the number and size of beta-galactosidase-positive (beta-gal+) clones were recorded. Cells isolated from somites I-V (the last five somites to have formed) or from unsegmented paraxial mesoderm did not give rise to any beta-gal+ clones. Cells isolated from somites VI-X or from the forelimb bud gave rise to beta-gal+ clones, but only on feeder cells. Cells from somites XI or older gave rise to beta-gal+ clones independently of the substrate. However, when cells isolated from unsegmented paraxial mesoderm or somites I-V were cultured with nontransgenic cells from the trunk (including neural tube and notochord), differentiation occurred on condition that the cells were in a three-dimensional aggregate, even though their specific position in the somite had been lost. By culturing explants ranging in size from 1 to < 100 cells in the presence of an inhibitor of cell division, we determined that a minimal number of 30-40 cells is required for mesodermal cells to differentiate.
Authors:
G Cossu; R Kelly; S Di Donna; E Vivarelli; M Buckingham
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  92     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-04-20     Completed Date:  1995-04-20     Revised Date:  2012-02-27    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2254-8     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology, Pasteur Institute, Centre National de la Recherche Scientifique URS67, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation*
Crosses, Genetic
Embryo, Mammalian / physiology*
Embryonic and Fetal Development
Female
Male
Mesoderm / cytology,  physiology*
Mice
Mice, Inbred Strains
Mice, Transgenic
Muscles / cytology*,  embryology
Myosins / biosynthesis,  genetics
Promoter Regions, Genetic
beta-Galactosidase / biosynthesis
Grant Support
ID/Acronym/Agency:
A.013//Telethon
Chemical
Reg. No./Substance:
EC 3.2.1.23/beta-Galactosidase; EC 3.6.4.1/Myosins
Comments/Corrections

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