| MyoD converts primary dermal fibroblasts, chondroblasts, smooth muscle, and retinal pigmented epithelial cells into striated mononucleated myoblasts and multinucleated myotubes. | |
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MedLine Citation:
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PMID: 2172969 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Shortly after their birth, postmitotic mononucleated myoblasts in myotomes, limb buds, and conventional muscle cultures elongate and assemble a cohort of myofibrillar proteins into definitively striated myofibrils. MyoD induces a number of immortalized and/or transformed nonmuscle cells to express desmin and several myofibrillar proteins and to fuse into myosacs. We now report that MyoD converts normal dermal fibroblasts, chondroblasts, gizzard smooth muscle, and pigmented retinal epithelial cells into elongated postmitotic mononucleated striated myoblasts. The sarcomeric localization of antibodies to desmin, alpha-actinin, titin, troponin-I, alpha-actin, myosin heavy chain, and myomesin in these converted myoblasts are indistinguishable from in vivo and in vitro normal myoblasts. Converted myoblasts fuse into typical anisodiametric multinucleated myotubes that often contract spontaneously. Conversion and subsequent expression of the skeletal myogenic program are autonomous events, occurring in four nonmuscle microenvironments consisting of different combinations of foreign extracellular matrix molecules. Early events associated with conversion by MyoD involve (i) withdrawal from the cell cycle, (ii) down-regulation of the subverted cell's ongoing differentiation program, and (iii) initiation of desmin synthesis in presumptive myoblasts and dramatic redistribution of microtubules and desmin intermediate filaments in postmitotic myoblasts. |
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Authors:
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J Choi; M L Costa; C S Mermelstein; C Chagas; S Holtzer; H Holtzer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 87 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1990 Oct |
Date Detail:
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Created Date: 1990-12-04 Completed Date: 1990-12-04 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7988-92 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Pennsylvania, Philadelphia 19104. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cartilage / cytology* Cell Differentiation Cell Division Cells, Cultured DNA Replication Fibroblasts / cytology Gizzard Muscle Proteins / genetics, physiology* Muscle, Smooth / cytology* MyoD Protein Pigment Epithelium of Eye / cytology* Skin / cytology* Transfection* |
| Grant Support | |
ID/Acronym/Agency:
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CA-18194/CA/NCI NIH HHS; HL-15835/HL/NHLBI NIH HHS; HL-37675/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Muscle Proteins; 0/MyoD Protein |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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