Document Detail


MyoD converts primary dermal fibroblasts, chondroblasts, smooth muscle, and retinal pigmented epithelial cells into striated mononucleated myoblasts and multinucleated myotubes.
MedLine Citation:
PMID:  2172969     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Shortly after their birth, postmitotic mononucleated myoblasts in myotomes, limb buds, and conventional muscle cultures elongate and assemble a cohort of myofibrillar proteins into definitively striated myofibrils. MyoD induces a number of immortalized and/or transformed nonmuscle cells to express desmin and several myofibrillar proteins and to fuse into myosacs. We now report that MyoD converts normal dermal fibroblasts, chondroblasts, gizzard smooth muscle, and pigmented retinal epithelial cells into elongated postmitotic mononucleated striated myoblasts. The sarcomeric localization of antibodies to desmin, alpha-actinin, titin, troponin-I, alpha-actin, myosin heavy chain, and myomesin in these converted myoblasts are indistinguishable from in vivo and in vitro normal myoblasts. Converted myoblasts fuse into typical anisodiametric multinucleated myotubes that often contract spontaneously. Conversion and subsequent expression of the skeletal myogenic program are autonomous events, occurring in four nonmuscle microenvironments consisting of different combinations of foreign extracellular matrix molecules. Early events associated with conversion by MyoD involve (i) withdrawal from the cell cycle, (ii) down-regulation of the subverted cell's ongoing differentiation program, and (iii) initiation of desmin synthesis in presumptive myoblasts and dramatic redistribution of microtubules and desmin intermediate filaments in postmitotic myoblasts.
Authors:
J Choi; M L Costa; C S Mermelstein; C Chagas; S Holtzer; H Holtzer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  87     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1990 Oct 
Date Detail:
Created Date:  1990-12-04     Completed Date:  1990-12-04     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7988-92     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Pennsylvania, Philadelphia 19104.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cartilage / cytology*
Cell Differentiation
Cell Division
Cells, Cultured
DNA Replication
Fibroblasts / cytology
Gizzard
Muscle Proteins / genetics,  physiology*
Muscle, Smooth / cytology*
MyoD Protein
Pigment Epithelium of Eye / cytology*
Skin / cytology*
Transfection*
Grant Support
ID/Acronym/Agency:
CA-18194/CA/NCI NIH HHS; HL-15835/HL/NHLBI NIH HHS; HL-37675/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Muscle Proteins; 0/MyoD Protein
Comments/Corrections

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