Document Detail


MyoD and Myf-5 differentially regulate the development of limb versus trunk skeletal muscle.
MedLine Citation:
PMID:  9428409     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The myogenic progenitors of epaxial (paraspinal and intercostal) and hypaxial (limb and abdominal wall) musculature are believed to originate in dorsal-medial and ventral-lateral domains, respectively, of the developing somite. To investigate the hypothesis that Myf-5 and MyoD have different roles in the development of epaxial and hypaxial musculature, we further characterized myogenesis in Myf-5- and MyoD-deficient embryos by several approaches. We examined expression of a MyoD-lacZ transgene in Myf-5 and MyoD mutant embryos to characterize the temporal-spatial patterns of myogenesis in mutant embryos. In addition, we performed immunohistochemistry on sectioned Myf-5 and MyoD mutant embryos with antibodies reactive with desmin, nestin, myosin heavy chain, sarcomeric actin, Myf-5, MyoD and myogenin. While MyoD(-/-) embryos displayed normal development of paraspinal and intercostal muscles in the body proper, muscle development in limb buds and brachial arches was delayed by about 2.5 days. By contrast, Myf-5(-/-) embryos displayed normal muscle development in limb buds and brachial arches, and markedly delayed development of paraspinal and intercostal muscles. Although MyoD mutant embryos exhibited delayed development of limb musculature, normal migration of Pax-3-expressing cells into the limb buds and normal subsequent induction of Myf-5 in myogenic precursors was observed. These results suggest that Myf-5 expression in the limb is insufficient for the normal progression of myogenic development. Taken together, these observations strongly support the hypothesis that Myf-5 and MyoD play unique roles in the development of epaxial and hypaxial muscle, respectively.
Authors:
B Kablar; K Krastel; C Ying; A Asakura; S J Tapscott; M A Rudnicki
Related Documents :
1436949 - Fine structure of developed human tongue muscle.
7956819 - The intracellular domain of mouse notch: a constitutively activated repressor of myogen...
15822819 - Increase of cardiotrophin-1 immunoreactivity in regenerating and overloaded but not den...
3401519 - Muscular dysgenesis in fowl: ultrastructural study of skeletal muscles in the crooked n...
18922599 - Sirt1 increases skeletal muscle precursor cell proliferation.
22151709 - Heart rate control via vagus nerve stimulation.
18274259 - Structure of skeletal muscles in leghorn type chicken from conservative and parent flocks.
15908269 - Motor unit synchronization between medial and lateral vasti muscles.
3001789 - Some effects of orthovanadate on smooth muscle cells of guinea-pig taenia caeci.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  124     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-02-02     Completed Date:  1998-02-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4729-38     Citation Subset:  IM    
Affiliation:
Institute for Molecular Biology and Biotechnology, Cancer Research Group, McMaster University, Hamilton, Ontario, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
DNA-Binding Proteins / genetics,  metabolism
Extremities / embryology*
Gene Expression Regulation, Developmental
Mice
Mice, Mutant Strains
Mice, Transgenic
Muscle Proteins / physiology*
Muscle, Skeletal / embryology*,  metabolism
MyoD Protein / physiology*
Myogenic Regulatory Factor 5
Paired Box Transcription Factors
Spine / embryology*
Stem Cells / physiology
Trans-Activators*
Transcription Factors*
Transgenes
beta-Galactosidase / genetics,  metabolism
Chemical
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Muscle Proteins; 0/Myf5 protein, mouse; 0/MyoD Protein; 0/Myogenic Regulatory Factor 5; 0/Paired Box Transcription Factors; 0/Trans-Activators; 0/Transcription Factors; 138016-91-8/Pax3 protein, mouse; EC 3.2.1.23/beta-Galactosidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  SpMyb functions as an intramodular repressor to regulate spatial expression of CyIIIa in sea urchin ...
Next Document:  Sizzled: a secreted Xwnt8 antagonist expressed in the ventral marginal zone of Xenopus embryos.