Document Detail

MyoD and Myf-5 differentially regulate the development of limb versus trunk skeletal muscle.
MedLine Citation:
PMID:  9428409     Owner:  NLM     Status:  MEDLINE    
The myogenic progenitors of epaxial (paraspinal and intercostal) and hypaxial (limb and abdominal wall) musculature are believed to originate in dorsal-medial and ventral-lateral domains, respectively, of the developing somite. To investigate the hypothesis that Myf-5 and MyoD have different roles in the development of epaxial and hypaxial musculature, we further characterized myogenesis in Myf-5- and MyoD-deficient embryos by several approaches. We examined expression of a MyoD-lacZ transgene in Myf-5 and MyoD mutant embryos to characterize the temporal-spatial patterns of myogenesis in mutant embryos. In addition, we performed immunohistochemistry on sectioned Myf-5 and MyoD mutant embryos with antibodies reactive with desmin, nestin, myosin heavy chain, sarcomeric actin, Myf-5, MyoD and myogenin. While MyoD(-/-) embryos displayed normal development of paraspinal and intercostal muscles in the body proper, muscle development in limb buds and brachial arches was delayed by about 2.5 days. By contrast, Myf-5(-/-) embryos displayed normal muscle development in limb buds and brachial arches, and markedly delayed development of paraspinal and intercostal muscles. Although MyoD mutant embryos exhibited delayed development of limb musculature, normal migration of Pax-3-expressing cells into the limb buds and normal subsequent induction of Myf-5 in myogenic precursors was observed. These results suggest that Myf-5 expression in the limb is insufficient for the normal progression of myogenic development. Taken together, these observations strongly support the hypothesis that Myf-5 and MyoD play unique roles in the development of epaxial and hypaxial muscle, respectively.
B Kablar; K Krastel; C Ying; A Asakura; S J Tapscott; M A Rudnicki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  124     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-02-02     Completed Date:  1998-02-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  4729-38     Citation Subset:  IM    
Institute for Molecular Biology and Biotechnology, Cancer Research Group, McMaster University, Hamilton, Ontario, Canada.
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MeSH Terms
DNA-Binding Proteins / genetics,  metabolism
Extremities / embryology*
Gene Expression Regulation, Developmental
Mice, Mutant Strains
Mice, Transgenic
Muscle Proteins / physiology*
Muscle, Skeletal / embryology*,  metabolism
MyoD Protein / physiology*
Myogenic Regulatory Factor 5
Paired Box Transcription Factors
Spine / embryology*
Stem Cells / physiology
Transcription Factors*
beta-Galactosidase / genetics,  metabolism
Reg. No./Substance:
0/DNA-Binding Proteins; 0/Muscle Proteins; 0/Myf5 protein, mouse; 0/MyoD Protein; 0/Myogenic Regulatory Factor 5; 0/Paired Box Transcription Factors; 0/Trans-Activators; 0/Transcription Factors; 138016-91-8/Pax3 protein, mouse; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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