Document Detail

Myeloperoxidase gene expression in infant leukemia: a Pediatric Oncology Group Study.
MedLine Citation:
PMID:  9638984     Owner:  NLM     Status:  MEDLINE    
A high incidence of co-expression of myeloid-associated antigens in infant B-precursor Acute Lymphocytic Leukemia (B-ALL) has been reported, but the significance of this finding is uncertain. To further assess myeloid differentiation and its prognostic significance in this disease, we investigated the frequency of myeloperoxidase (MPO) gene expression in the blast cells from 43 infants with B-ALL registered in a Pediatric Oncology Group (POG) Pilot Study for Treatment of Infant ALL, utilizing a molecular probe for detection of MPO messenger RNA (mRNA) by Northern blot hybridization and a monoclonal antibody to detect MPO-protein by immunohistochemical staining. Sufficient RNA for Northern blot was extracted from 32 bone marrow or blood samples. In two cases, MPO mRNA was determined by a reverse transcriptase-polymerase chain reaction assay and was negative in both cases. MPO-specific transcripts (MPO+) were present in 19 of 34 (56%) samples analyzed. Immunoreactive MPO protein was positive in 13 of the 20 (65%) patients studied. No correlation was found between MPO gene expression and clinical or laboratory features, karyotypic patterns or clinical outcome. The high frequency of MPO gene expression demonstrated in this study suggests that leukemogenic events in many cases of infant B-ALL appear to involve a pluripotent stem cell not yet fully committed to lymphoid differentiation.
C S Alvarado; G E Austin; M J Borowitz; J J Shuster; A J Carroll; E D Austin; M Zhou; S R Zaki; J Pullen
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Publication Detail:
Type:  Clinical Trial; Controlled Clinical Trial; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Leukemia & lymphoma     Volume:  29     ISSN:  1042-8194     ISO Abbreviation:  Leuk. Lymphoma     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-09-08     Completed Date:  1998-09-08     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9007422     Medline TA:  Leuk Lymphoma     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  145-60     Citation Subset:  IM    
Emory University School of Medicine, Atlanta, Georgia, USA.
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MeSH Terms
Antibodies, Monoclonal
Antineoplastic Combined Chemotherapy Protocols / therapeutic use
Burkitt Lymphoma / drug therapy,  genetics*
DNA Primers
Gene Expression Regulation, Enzymologic / physiology*
Gene Expression Regulation, Neoplastic / physiology*
Infant, Newborn
Peroxidase / genetics*
Pilot Projects
Treatment Outcome
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/DNA Primers; EC

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